Comparative molecular cytogenetics of anurans of the genus Pseudis with emphasis on the study of sex chromosomes

• Grant number: 14/23542-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2015
• End date: January 31, 2017
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Luciana Bolsoni Lourenço
• Grantee: Luciana Bolsoni Lourenço
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The hylid genera Pseudis and Lysapsus together comprise eleven South American species, and all of them were already karyotyped. One remarkable cytogenetic feature of this anuran group is the presence of heteromorphic sex chromosomes Z and W in Pseudis tocantins. Based on the chromosomal morphology and NOR location, previous studies suggested that the sex chromosomes of P. tocantins is homeologous to chromosome 5 of P. cardosoi and chromosome 7 of the remaining species of Pseudis and Lysapsus. Recent researches showed that the heterochromatic blocks of the Z and W chromosomes of P. tocantins differ not only in size, but also in composition. Sequences of the satellite DNA PcP190EcoRI were found in both sex chromosomes, but the sequence mapped by FISH on Wq greatly differed from that which was isolated from microdissected Z chromosomes. Such data suggest an important role of this satellite DNA in the differentiation of these Z and W chromosomes. Despite these intriguing findings regarding P. tocantins, the sex chromosomes of Lysapsus and the remaining species of Pseudis remain unknown. In the present proposal, we will investigate about the presence of the satellite DNA PcP190EcoRI in representative species of the genera Pseudis and Lysapsus, aiming to evaluate the possible involvement of these DNA sequences in the karyotype evolution in this group. We will also employ the comparative genomic hybridization (CGH) in an attempt to identify the sex chromosomes of P. fusca, P. minuta, P. bolbodactyla and of one representative species of the genus Lysapsus. Additionally, we propose the sequencing of the sex chromosomes of P. tocantins, aiming to search for W-specific sequences, including candidate genes for sex determination in this species. Therefore, with the present proposal we intend to provide relevant advance in the study of the sex chromosomes of P. tocantins. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GATTO, KALEB PRETTO; SEGER, KARIN REGINA; DE ANCHIETA GARCIA, PAULO CHRISTIANO; LOURENCO, LUCIANA BOLSONI. Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs. GENES, v. 10, n. 2, FEB 2019. (14/23542-6)

GATTO, KALEB PRETTO; SMITH, JERAMIAH J.; LOURENCO, LUCIANA BOLSONI. The mitochondrial genome of the endemic Brazilian paradoxical frog Pseudis tocantins (Hylidae). MITOCHONDRIAL DNA PART B-RESOURCES, v. 3, n. 2, p. 1106-1107, 2018. (14/23542-6)

GATTO, KALEB P.; MATTOS, V, JOAO; SEGER, KARIN R.; LOURENCO, LUCIANA B.. Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements. FRONTIERS IN GENETICS, v. 9, AUG 7 2018. (14/23542-6)

GATTO, KALEB PRETTO; BUSIN, CARMEN SILVIA; LOURENCO, LUCIANA BOLSONI. Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins. PLoS One, v. 11, n. 5, MAY 23 2016. (11/05416-5, 14/23542-6)