| Full text | |
| Author(s): |
Silva, Meiricris T.
[1]
;
Nascimento, Tabata L.
[1]
;
Pereira, Marcelo G.
[1]
;
Siqueira, Adriane S.
[2]
;
Brum, Patricia C.
[3]
;
Jaeger, Ruy G.
[2]
;
Miyabara, Elen H.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Ave Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Phys Educ & Sport, Dept Biodynam, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Cell and Tissue Research; v. 365, n. 1, p. 173-186, JUL 2016. |
| Web of Science Citations: | 0 |
| Abstract | |
We investigated the role of beta 2-adrenoceptors in the connective tissue remodeling of regenerating muscles from beta 2-adrenoceptor knockout (beta 2KO) mice. Tibialis anterior muscles from beta 2KO mice were cryolesioned and analyzed after 3, 10, and 21 days. Regenerating muscles from beta 2KO mice showed a significant increase in the area density of the connective tissue and in the amount of collagen at 10 days compared with wild-type (WT) mice. A greater increase occurred in the expression levels of collagen I, III, and IV in regenerating muscles from beta 2KO mice evaluated at 10 days compared with WT mice; this increase continued at 21 days, except for collagen III. Matrix metalloproteinase (MMP-2) activity increased to a similar extent in regenerating muscles from both beta 2KO and WT mice at 3 and 10 days. This was also the case for MMP-9 activity in regenerating muscles from both beta 2KO and WT mice at 3 days; however, at 10 days post-cryolesion, this activity returned to baseline levels only in WT mice. MMP-3 activity was unaltered in regenerating muscles at 10 days. mRNA levels of tumor necrosis factor-alpha increased in regenerating muscles from WT and beta 2KO mice at 3 days and, at 10 days post-cryolesion, returned to baseline only in WT mice. mRNA levels of interleukin-6 increased in muscles from WT mice at 3 days post-cryolesion and returned to baseline at 10 days post-cryolesion but were unchanged in beta 2KO mice. Our results suggest that the beta 2-adrenoceptor contributes to collagen remodeling during muscle regeneration by decreasing MMP-9 activity. (AU) | |
| FAPESP's process: | 14/13874-1 - Skeletal muscle regeneration in mice with Marfan Syndrome: study of the regenerative potential of satellite cells |
| Grantee: | Meiricris Tomaz da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/23391-8 - Regenerative response of skeletal muscles from mice with Marfan Syndrome |
| Grantee: | Elen Haruka Miyabara |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/04783-0 - The influence of heat shock proteins in the recovery of skeletal muscle of old mice |
| Grantee: | Tábata Leal Nascimento |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/21238-2 - Skeletal muscle plasticity after injury: role of beta2-adrenoceptor |
| Grantee: | Elen Haruka Miyabara |
| Support Opportunities: | Regular Research Grants |