Tracheal occlusion and ventilation changes the nitric oxide pathway in congenital diaphragmatic hernia model

Author(s)

Lanhellas Goncalves, Frances Lilian ^[1] ; Figueira, Rebeca Lopes ^[1] ; Gallindo, Rodrigo Melo ^[1] ; Bertoncini Simoes, Ana Leda ^[1] ; Coleman, Alan ^[2] ; Peiro, Jose Luis ^[2] ; Sbragia, Lourenco ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Pediat Surg, Lab Expt Fetal Surg, Ribeirao Preto, SP - Brazil

^[2] Div Cincinnati Childrens Hosp Med Ctr, Pediat Gen & Thorac Surg, Cincinnati, OH - USA

• Document type: Journal article
• Source: JOURNAL OF SURGICAL RESEARCH; v. 203, n. 2, p. 466-475, JUN 15 2016.
• Web of Science Citations: 1

Abstract

Background: Congenital diaphragmatic hernia (CDH) is associated with lung hypoplasia and pulmonary hypertension. Tracheal occlusion (TO) stimulates fetal lung growth and maturation and reverse vascular changes responsible for pulmonary hypertension, which are related to mechanisms involving nitric oxide (NO) in CDH. We aim to evaluate the effect of TO and ventilation on NO pathways. Methods: Eight groups were created: (1) control; (2) control ventilated (CV); (3) CDH (CDH); (4) CDH ventilated (CDHV); (5) TO control; (6) TO ventilated; (7) TO + CDH; and (8) TO + CDH ventilated (CDHTOV). Fetuses were weighed, and volume ventilated for 30 min after harvested. Total lung weight and the ratio of total lung weight to body weight, thickness of the middle layer of the pulmonary arteriole, and the air space diameter were measured. The NO synthase inducible and NO synthase inducible were performed by immunohistochemistry and Western blotting. Results: The total lung weight and the ratio of total lung weight to body weight decreased in animals with nitrofen and also after ventilation for all groups (P < 0.05). The thickness of the middle layer of the pulmonary arteriole decreased in all groups with TO when compared with controls (P < 0.001). The air space diameter decreased after ventilation in the CDHTOV compared to the TO + nitrofeneinduced CDH (P < 0.001). Compared to nonventilated cohorts, NO synthase inducible increased in CV and TO ventilated (P < 0.001) and decreased in CDHV and CDHTOV (P < 0.001). NO synthase inducible increased in CV and CDHV (P < 0.001) and decreased in the TO control and CDHTOV (P < 0.001). Conclusions: TO and ventilation alter the NO pathway with possible implications in reducing the pulmonary hypertension in CDH. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats
• Grantee: Frances Lilian Lanhellas Gonçalves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen
• Grantee: Lourenço Sbragia Neto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02519-6 - Evaluation of myocardial contractility and myocyte function of neonate rabbits with congenital diaphragmatic hernia created surgically
• Grantee: Lourenço Sbragia Neto
• Support Opportunities: Scholarships abroad - Research