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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in Sao Paulo, Brazil

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Betiol, Julio C. [1, 2] ; Sichero, Laura [2, 1, 3] ; de Olival Costa, Henrique O. [4] ; de Matos, Leandro L. [5] ; Andreoli, Maria A. [6] ; Ferreira, Silvaneide [1, 2] ; Faraj, Sheila F. [7] ; de Mello, Evandro S. [7] ; Sobrinho, Joao S. [1, 2] ; Brandao, Lenine G. [5] ; Cernea, Claudio R. [5] ; Kulcsar, Marco A. [5] ; Pinto, Fabio R. [5] ; Goncalves, Antonio J. [8] ; Menezes, Marcelo B. [8] ; Silva, Leonardo [4] ; Rossi, Lia M. [4] ; Lima Nunes, Rafaella A. [6] ; Termini, Lara [6] ; Villa, Luisa L. [9, 2, 1, 6]
Total Authors: 20
Affiliation:
[1] Univ Sao Paulo, Fac Med, Mol Biol Lab, Hosp Clin, Ctr Translat Res Oncol, Inst Canc Estado Sao Paulo, Sao Paulo - Brazil
[2] ICESP, Sao Paulo - Brazil
[3] Ctr Translat Oncol ICESP, Av Dr Arnaldo 251, 8 Andar, BR-01246000 Sao Paulo, SP - Brazil
[4] Sch Med FCMSCSP, Dept Otolaringol, Santa Casa Sao Paulo, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Head & Neck Surg, Sao Paulo - Brazil
[6] Sch Med FCMSCSP, HPV Inst, Santa Casa Sao Paulo, Sao Paulo - Brazil
[7] Canc Inst Sao Paulo ICESP, Dept Pathol, Sao Paulo - Brazil
[8] Sch Med FCMSCSP, Dept Surg, Santa Casa Sao Paulo, Sao Paulo - Brazil
[9] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Sao Paulo - Brazil
Total Affiliations: 9
Document type: Journal article
Source: INFECTIOUS AGENTS AND CANCER; v. 11, MAY 4 2016.
Web of Science Citations: 5
Abstract

Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in Sao Paulo. Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry. Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors. Conclusion: Our results support a role for HPV-16 in a subset of HNSCC. (AU)

FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
Grantee:Luisa Lina Villa
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/09616-9 - HPV diversity and cancer risk
Grantee:Luisa Lina Villa
Support type: Regular Research Grants
FAPESP's process: 12/01513-9 - HPV diversity and cancer risk
Grantee:Julio Cesar Betiol
Support type: Scholarships in Brazil - Master