EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma

Author(s):Show less -

Flores, Isadora L. ^{[1, 2]} ; Kawahara, Rebeca ^[1] ; Miguel, Marcia C. C. ^{[2, 3]} ; Granato, Daniela C. ^[1] ; Domingues, Romenia R. ^[1] ; Macedo, Carolina C. S. ^{[1, 2]} ; Carnielli, Carolina M. ^[1] ; Yokoo, Sami ^[1] ; Rodrigues, Priscila C. ^[2] ; Monteiro, Barbara V. B. ^{[2, 3]} ; Oliveira, Carine E. ^[2] ; Salmon, Cristiane R. ^[4] ; Nociti, Francisco H. ^[4] ; Lopes, Marcio A. ^[2] ; Santos-Silva, Alan ^[2] ; Winck, Flavia V. ^[1] ; Coletta, Ricardo D. ^[2] ; Paes Leme, Adriana F. ^[1]

Affiliation

^[1] CNPEM, LNBio, Lab Nacl Biociencias, Lab Espectrometria Massas, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Fac Odontol Piracicaba, Dept Diagnost Oral, Piracicaba, SP - Brazil

^[3] Univ Fed Rio Grande do Norte, Dept Odontol, BR-59072970 Natal, RN - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Fac Odontol Piracicaba, Dept Protese & Periodontia, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: Clinical Science; v. 130, n. 10, p. 785-799, MAY 1 2016.
• Web of Science Citations: 12

Abstract

EEF1D (eukaryotic translation elongation factor 1 delta) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial-mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT. (AU)

• FAPESP's process: 11/22421-2 - Determination of cleavage sites of recombinant ADAM-17 in human cells
• Grantee: Rebeca Kawahara Sakuma
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 09/53839-2 - Creation of a Digital Pathology Laboratory using a histological slidescanner
• Grantee: Oslei Paes de Almeida
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 09/54067-3 - Acquisition of a mass spectrometer coupled to a liquid chromatography system for increasing the capacity to meet the needs of users and for making new technologies available in the Laboratory of Mass Spectrometry
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/01607-6 - The biological role of EEF1D, fascin and plectin on oral squamous cell carcinoma
• Grantee: Ricardo Della Coletta
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/19278-0 - Study of regulation of ADAMs in oral cancer
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Research Grants - Young Investigators Grants