Resveratrol-Loaded Liquid-Crystalline System Inhibits UVB-Induced Skin Inflammation and Oxidative Stress in Mice

Author(s)

Fujimura, Andressa T. ^[1] ; Martinez, Renata M. ^[2] ; Pinho-Ribeiro, Felipe A. ^[3] ; Lopes Dias da Silva, Amelia M. ^[4] ; Baracat, Marcela M. ^[2] ; Georgetti, Sandra R. ^[2] ; Verri, Jr., Waldiceu A. ^[3] ; Chorilli, Marlus ^[1] ; Casagrande, Rubia ^[2]

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Ciencias Farmaceut, Rodovia Araraquara Jau, Km 01, BR-14801902 Araraquara, Sao Paulo - Brazil

^[2] Univ Estadual Londrina, Univ Hosp, Dept Ciencias Farmaceut, Avenida Robert Koch 60, BR-86039440 Londrina, Parana - Brazil

^[3] Univ Estadual Londrina, Dept Ciencias Patol, Rodovia Celso Garcia Cid, Km 380, PR445, BR-86057970 Londrina, Parana - Brazil

^[4] Univ Tras Os Montes & Alto Douro UTAD, Ctr Invest & Tecnol Ciencias Agroambientais & Bio, Quinta Prados 1013, P-5001801 Vila Real - Portugal

• Document type: Journal article
• Source: Journal of Natural Products; v. 79, n. 5, p. 1329-1338, MAY 2016.
• Web of Science Citations: 15

Abstract

Evidence shows beneficial effects of resveratrol (RES) on human health. However, its poor aqueous solubility limits therapeutic effectiveness. Thus, the use of nano structured delivery systems for RES, such as a liquid-crystalline system (LCS), could be viable. The purpose of this study was to develop, characterize, and determine the in vivo effectiveness of a RES-loaded LCS. We studied an LCS containing silicon glycol copolymer, polyether functional siloxane, and the polymeric dispersion carbomer homopolymer type B (C974) in the ratio 20:55:25 with and without RES. Results obtained using polarized light microscopy, small-angle X-ray scattering, and rheology analysis showed that the RES-loaded LCS system presents a lamellar structure and behaves as a non-Newtonian fluid presenting pseudoplastic (the apparent viscosity decreases as the stress increases) and thixotropic (the apparent viscosity decreases with the duration of stress) behaviors. Cytotoxicity studies showed that the formulation components are noncytotoxic. Topical application of a RES-loaded LCS protected hairless mice from UVB-irradiation-induced skin damage by inhibiting edema, neutrophil recruitment, lipid hydroperoxide and superoxide anion production, gp9lphox mRNA expression, and oxidative stress. The RES-loaded LCS maintained 2,2'-azinobis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing abilities, catalase activity, reduced glutathione levels, and mRNA expression of glutathione peroxidase 1 and glutathione recuctase. The RES-loaded LCS also up-regulated matrix metalloproteinase-9 activity, IL-10 production, and mRNA expression of transcription factor Nrf2 and heme oxygenase-1. Therefore, a RES-loaded LCS is a promising new therapeutic approach to mitigate skin photodamage. (AU)

• FAPESP's process: 14/24180-0 - Liquid crystalline systems as potential strategy ofr incorporation of trans-resveratrol: development, physicochemical characterization and in vitro and in vivo biological assays
• Grantee: Marlus Chorilli
• Support type: Regular Research Grants
• FAPESP's process: 12/16956-3 - Development and characterization of liquid crystalline systems for incorporation of trans-resveratrol: in vitro cutaneous permeation tests and in vivo assessment of the effectiveness
• Grantee: Andressa Terumi Fujimura
• Support type: Scholarships in Brazil - Master