Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model

Author(s)

Curra, Claudia ^{[1, 2]} ; Cardoso, Camila Lopes ^{[1, 3, 2]} ; Ferreira Junior, Osny ^{[1, 3, 2]} ; Curi, Marcos Martins ^{[1, 2]} ; Matsumoto, Mariza Akemi ^{[1, 2, 4]} ; Cavenago, Bruno Cavalini ^{[1, 2, 5]} ; dos Santos, Pamela Leticia ^{[1, 2]} ; Santiago Junior, Joel Ferreira ^{[1, 2, 6]}

Affiliation

^[1] Univ Sagrado Coracao, Dept Oral Surg, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Dept Oral Surg, Bauru, SP - Brazil

^[3] Univ Sagrado Coracao, Dept Oral Surg, Bauru Dent Sch, Bauru, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Aracatuba, SP - Brazil

^[5] Univ Sao Paulo, Bauru Dent Sch, Dept Endodont, Bauru, SP - Brazil

^[6] Univ Sao Paulo, Bauru Dent Sch, Dept Implantol, Bauru, SP - Brazil

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 31, n. 5, p. 308-313, MAY 2016.
• Web of Science Citations: 4

Abstract

PURPOSE: To evaluate a modified experimental model for medication-related osteonecrosis of the jaw (MRONJ) through the upper right central incisor extraction followed by intravenous bisphosphonate administration. METHODS: Forty five rats underwent the upper right central incisor tooth extraction were divided in 2 groups: Group I - experimental group, 30 rats received an intravenous administration protocol of zoledronic acid 35 mu g/kg into the tail vein every two weeks, totalizing four administrations, during eight weeks of administration, previously the extraction, and Group II - control group, 15 rats didn't received any medication before extraction. The groups were subdivided in postoperative periods: 14/28/42 days. Clinical analysis and microtomography were performed to verify the presence of osteonecrosis. In addition, descritive histological analysis of hematoxylineosin stained sections was performed to evaluate the presence of osteonecrosis or necrotic foci. RESULTS: Twelve (40%) rats, from experimental group, showed clinical signs of MRONJ (p=0.005), however, all samples showed imaginologic findings like osteolysis and loss of integrity of the cellular walls (p <= 0.001). Microscopic evaluation revealed osteonecrosis areas with microbial colonies and inflammatory infiltrate (p <= 0.001). In the control group, all animals presented the chronology of a normal wound healing. CONCLUSIONS: The presence of medication-related osteonecrosis of the jaw after maxillary central incisor extraction in rats. This new experimental model may be considered an option for the study of MRONJ. (AU)

• FAPESP's process: 14/10693-6 - Experimental evaluation of platelet-rich plasma in the treatment of bisphosphonate-related osteonecrosis of the jaw
• Grantee: Camila Lopes Cardoso
• Support Opportunities: Regular Research Grants