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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the gene encoding component C3 of the complement system from the spider Loxosceles laeta venom glands: Phylogenetic implications

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Author(s):
Myamoto, D. T. [1] ; Pidde-Queiroz, G. [1] ; Pedroso, A. [1] ; Goncalves-de-Andrade, R. M. [1] ; van den Serg, C. W. [2] ; Tambourgi, D. V. [1]
Total Authors: 6
Affiliation:
[1] Butantan Inst, Immunochem Lab, Av Vital Brasil 1500, Sao Paulo, SP - Brazil
[2] Cardiff Univ, Inst Mol & Expt Med, Sch Med, Cardiff, S Glam - Wales
Total Affiliations: 2
Document type: Journal article
Source: Immunobiology; v. 221, n. 9, p. 953-963, SEP 2016.
Web of Science Citations: 3
Abstract

A transcriptome analysis of the venom glands of the spider Loxosceles laeta, performed by our group, in a previous study (Fernandes-Pedrosa et al., 2008), revealed a transcript with a sequence similar to the human complement component C3. Here we present the analysis of this transcript. cDNA fragments encoding the C3 homologue (Lox-C3) were amplified from total RNA isolated from the venom glands of L laeta by RACE-PCR. Lox-C3 is a 5178 bps cDNA sequence encoding a 190 kDa protein, with a domain configuration similar to human C3. Multiple alignments of C3-like proteins revealed two processing sites, suggesting that Lox-C3 is composed of three chains. Furthermore, the amino acids consensus sequences for the thioester was found, in addition to putative sequences responsible for FB binding. The phylogenetic analysis showed that Lox-C3 belongs to the same group as two C3 isoforms from the spider Hasarius adansoni (Family Salcitidae), showing 53% homology with these. This is the first characterization of a Loxosceles cDNA sequence encoding a human C3 homologue, and this finding, together with our previous finding of the expression of a FB-like molecule, suggests that this spider species also has a complement system. This work will help to improve our understanding of the innate immune system in these spiders and the ancestral structure of C3. (C) 2016 Elsevier GmbH. All rights reserved (AU)

FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC