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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

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Author(s):
de Moraes, Marcela Cristina ; Cardoso, Carmen Lucia ; Seidl, Claudia ; Moaddel, Ruin ; Cass, Quezia Bezerra
Total Authors: 5
Document type: Review article
Source: CURRENT PHARMACEUTICAL DESIGN; v. 22, n. 39, p. 5976-5987, 2016.
Web of Science Citations: 9
Abstract

Affinity-based chromatography assays encompass the use of solid supports containing immobilized biological targets to monitor binding events in the isolation, identification and/or characterization of bioactive compounds. This powerful bioanalytical technique allows the screening of potential binders through fast analyses that can be directly performed using isolated substances or complex matrices. An overview of the recent researches in frontal and zonal affinity-based chromatography screening assays, which has been used as a tool in the identification and characterization of new anti-cancer agents, is discussed. In addition, a critical evaluation of the recently emerged ligands fishing assays in complex mixtures is also discussed. (AU)

FAPESP's process: 13/01710-1 - Enzyme ligand: new models of screening
Grantee:Quezia Bezerra Cass
Support Opportunities: Research Projects - Thematic Grants