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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Organization of some repetitive DNAs and B chromosomes in the grasshopper Eumastusia koebelei koebelei (Rehn, 1909) (Orthoptera, Acrididae, Leptysminae)

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Author(s):
Anjos, Allison ; Loreto, Vilma ; Cabral-de-Mello, Diogo C.
Total Authors: 3
Document type: Journal article
Source: COMPARATIVE CYTOGENETICS; v. 10, n. 2, p. 219-228, 2016.
Web of Science Citations: 3
Abstract

B chromosomes occur in approximately 15% of eukaryotes and are usually heterochromatic and rich in repetitive DNAs. Here we describe characteristics of a B chromosome in the grasshopper Eumastusia koebelei koebelei (Rehn, 1909) through classical cytogenetic methods and mapping of some repetitive DNAs, including multigene families, telomeric repeats and a DNA fraction enriched with repetitive DNAs obtained from DOP-PCR. Eumastusia k. koebelei presented 2n=23, X0 and, in one individual, two copies of the same variant of a B chromosome were noticed, which are associated during meiosis. The C-positive blocks were located in the pericentromeric regions of the standard complement and along the entire length of the B chromosomes. Some G+C-rich heterochromatic blocks were noticed, including conspicuous blocks in the B chromosomes. The mapping of 18S rDNA and U2 snDNA revealed only autosomal clusters, and the telomeric probe hybridized in terminal regions. Finally, the DOP-PCR probe obtained from an individual without a B chromosome revealed signals in the heterochromatic regions, including the entire length of the B chromosome. The possible intraspecific origin of the B chromosomes, due to the shared pool of repetitive DNAs between the A and B chromosomes and the possible consequences of their association are discussed. (AU)

FAPESP's process: 14/06226-3 - Karyotypic structure and evolution among Cercopidae splitebug species (Hemiptera: Auchenorrhyncha) based in the analysis of repetitive DNAs
Grantee:Allison Kleiton do Anjos
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/11763-8 - Contributions to understanding of structure and evolution of sex chromosome systems using as models grashopper and cricket species
Grantee:Diogo Cavalcanti Cabral de Mello
Support Opportunities: Regular Research Grants