| Full text | |
| Author(s): |
Oliveira, Luciana Coutinho
;
Souza, Diorge Paulo
;
Oka, Gabriel Umaji
;
Lima, Filipe da Silva
;
Oliveira, Ronaldo Junio
;
Favaro, Denize Cristina
;
Wienk, Hans
;
Boelens, Rolf
;
Farah, Chuck Shaker
;
Salinas, Roberto Kopke
Total Authors: 10
|
| Document type: | Journal article |
| Source: | Structure; v. 24, n. 10, p. 1707-1718, OCT 4 2016. |
| Web of Science Citations: | 3 |
| Abstract | |
The type IV secretion system (T4SS) from the phytopathogen Xanthomonas citri (Xac) is a bactericidal nanomachine. The T4SS core complex is a ring composed of multiple copies of VirB7-VirB9-VirB10 subunits. Xac-VirB7 contains a disordered N-terminal tail (VirB7(NT)) that recognizes VirB9, and a C-terminal domain (VirB7(CT)) involved in VirB7 self-association. Here, we show that VirB7(NT) forms a short beta strand upon binding to VirB9 and stabilizes it. A tight interaction between them is essential for T4SS assembly and antibacterial activity. Abolishing VirB7 self-association or deletion of the VirB7 C-terminal domain impairs this antibacterial activity without disturbing T4SS assembly. These findings reveal protein interactions within the core complex that are critical for the stability and activity of a T4SS. (AU) | |
| FAPESP's process: | 13/17883-2 - Structure and dynamics of the Na+/Ca2+ exchanger from Drosophila melanogaster |
| Grantee: | Roberto Kopke Salinas |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/07777-5 - Cyclic di-GMP signaling and the Type IV macromolecule secretion system in Xanthomonas citri. |
| Grantee: | Shaker Chuck Farah |
| Support Opportunities: | Research Projects - Thematic Grants |