| Full text | |
| Author(s): |
Pitondo-Silva, Andre
;
Devechio, Beatriz Baptistella
;
Silva Moretto, Jessica Aparecida
;
Stehling, Eliana Guedes
Total Authors: 4
|
| Document type: | Journal article |
| Source: | Canadian Journal of Microbiology; v. 62, n. 10, p. 820-826, OCT 2016. |
| Web of Science Citations: | 4 |
| Abstract | |
This study investigated bacteria from soil samples to (i) determine the main bacterial genera and species having resistance to carbapenem and other beta-lactams and (ii) establish if the mechanism of resistance was due to the production of metallo-beta-lactamases. The isolates were characterized by PCR for metallo-beta-lactamases and integrons, by antimicrobial susceptibility testing, and by sequencing. The antimicrobial profile of 40 imipenem-resistant Gram-positive soil isolates from all Brazilian regions demonstrated that 31 (77.5%) of them were multidrug resistant. Among the 40 isolates, 19 presented the bla(VIM) gene and class 1 integrons by PCR. Six of the 19 isolates were identified as Paenibacillus sp., 12 as Bacillus sp., and just 1 was classified as Staphylococcus sp., by sequencing of the 16S rRNA gene. These results suggest that bacteria from soil can act as a source of bla(VIM-1) genes, representing a threat to public health. (AU) | |
| FAPESP's process: | 13/19401-5 - Research of genes involved in the resistance to carbapenems and monobactams in environmental isolates of Pseudomonas aeruginosa |
| Grantee: | Eliana Guedes Stehling |
| Support Opportunities: | Regular Research Grants |