Attenuated PGC-1 alpha Isoforms following Endurance Exercise with Blood Flow Restriction

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Conceicao, Miguel Soares ; Traina Chacon-Mikahil, Mara Patricia ; Telles, Guilherme Defante ; Libardi, Cleiton Augusto ; Mendes Junior, Edson Manoel ; Vechin, Felipe Cassaro ; Lugnani De Andrade, Andre Luis ; Gaspari, Arthur Fernandes ; Brum, Patricia Chakur ; Cavaglieri, Claudia Regina ; Serag, Sara ; Spiegelman, Bruce M. ; Hawley, John A. ; Camera, Donny M.

• Document type: Journal article
• Source: MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 48, n. 9, p. 1699-1707, SEP 2016.
• Web of Science Citations: 9

Abstract

Introduction: Exercise performed with blood flow restriction simultaneously enhances the acute responses to both myogenic and mitochondrial pathways with roles in training adaptation. We investigated isoform-specific gene expression of the peroxisome proliferator-activated receptor gamma coactivator 1 and selected target genes and proteins regulating skeletal muscle training adaptation. Methods: Nine healthy, untrained males participated in a randomized, counterbalanced, crossover design in which each subject completed a bout of low-intensity endurance exercise performed with blood flow restriction (15 min cycling at 40% of (v) over dot O-2peak, BFR-EE), endurance exercise (30min cycling at 70% of (v) over dot O-2peak, EE), or resistance exercise (4 x 10 repetitions of leg press at 70% of one-repetition maximum) separated by at least 1 wk of recovery. A single resting muscle biopsy (vastus lateralis) was obtained 2 wk before the first exercise trial (rest) and 3 h after each bout. Results: Total PGC-1 alpha mRNA abundance, along with all four isoforms, increased above rest with EE only (P < 0.05) being higher than BFR-EE (P < 0.05). PGC-1 alpha 1, 2, and 4 were higher after EE compared with resistance exercise (P < 0.05). EE also increased vascular endothelial growth factor, Hif-1 alpha, and MuRF-1 mRNA abundance above rest (P < 0.05), whereas COXIV mRNA expression increased with EE compared with BFR-EE (P < 0.05). Conclusion: The attenuated expression of all four PGC-1 alpha isoforms when EE is performed with blood flow restriction suggests this type of exercise provides an insufficient stimulus to activate the signaling pathways governing mitochondrial and angiogenesis responses observed with moderate-to high-intensity EE. (AU)

• FAPESP's process: 14/00985-0 - Effect of 12 weeks of concurrent training in the molecular signaling responses in older subjects
• Grantee: Miguel Soares Conceição
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate