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The effects of concurrent training on the epigenetic mechanism of DNA methylation

Grant number: 18/16513-0
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): November 01, 2018
Effective date (End): April 30, 2019
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Carlos Ugrinowitsch
Grantee:Guilherme Defante Telles
Supervisor abroad: John Alan Hawley
Home Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : Australian Catholic University, Melbourne (ACU), Australia  
Associated to the scholarship:17/01297-8 - Effects of concurrent training on the epigenetic mechanism of DNA methylation, BP.MS


The DNA Methylation Level responses in myogenic genes related to satellite cells (SCs) activation can be mechanisms that explain the lower muscle gains with Concurrent Training (CT) compared to Resistance Training (RT) alone, phenomenon known as Interference Effect. Aim: To determine and compare the methylation and the mRNA expression levels of the myogenic regulatory factors (MyoD, Myf5, MyoG, MRF4) and mitochondrial-related genes (PGC-1alpha, TFAM, PPAR-gamma) after Resistance Exercise (RE), Endurance Exercise (EE) and Concurrent Exercise (CE). Methods: 9 untrained young men participated in a randomized and balanced cross over experimental design. Each volunteer performed a bout of RE, EE and CE. The RE was composed by 2 exercises (Leg Press 45 and Leg extension), 2x8-12 maximum repetitions with 60s rest. The EE was performed on a treadmill, composed by 4 minutes warm up at 50% of the velocity reached on the maximum oxygen consumption (vVO2max) followed by 12 x 1 minute sprints at 100% of the vVO2max with 1 minute rest at 50% of vVO2max. The CE was composed by the same RE protocol followed by EE with 5 minutes rest between them. Twelve muscle samples were taken: immediately before, and 3 times after each exercise session (immediately (0h), 4 hours and 8 hours). Our hypothesis is that the methylation level of myogenic gene downregulation with CE will be lower than with RE, which could be one factor to induce the Interference Effect. (AU)