| Full text | |
| Author(s): Show less - |
Silva, Thaiomara A.
;
Smuczek, Basilio
;
Valadao, Iuri C.
;
Dzik, Luciana M.
;
Iglesia, Rebeca P.
;
Cruz, Mario C.
;
Zelanis, Andre
;
de Siqueira, Adriane S.
;
Serrano, Solange M. T.
;
Goldberg, Gary S.
;
Jaeger, Ruy G.
;
Freitas, Vanessa M.
Total Authors: 12
|
| Document type: | Journal article |
| Source: | ONCOTARGET; v. 7, n. 31, p. 49998-50016, AUG 2 2016. |
| Web of Science Citations: | 13 |
| Abstract | |
Extracellular vesicles play important roles in tumor development. Many components of these structures, including microvesicles and exosomes, have been defined. However, mechanisms by which extracellular vesicles affect tumor progression are not fully understood. Here, we investigated vesicular communication between mammary carcinoma cells and neighboring nontransformed mammary fibroblasts. Nonbiased proteomic analysis found that over 1% of the entire proteome is represented in these vesicles, with the neuroblast differentiation associated protein AHNAK and annexin A2 being the most abundant. In particular, AHNAK was found to be the most prominent component of these vesicles based on peptide number, and appeared necessary for their formation. In addition, we report here that carcinoma cells produce vesicles that promote the migration of recipient fibroblasts. These data suggest that AHNAK enables mammary carcinoma cells to produce and release extracellular vesicles that cause disruption of the stroma by surrounding fibroblasts. This paradigm reveals fundamental mechanisms by which vesicular communication between carcinoma cells and stromal cells can promote cancer progression in the tumor microenvironment. (AU) | |
| FAPESP's process: | 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 11/09472-7 - AHNAK regulates formation and exchange of extracellular vesicles between breast tumor cells and fibroblasts |
| Grantee: | Thaiomara Alves Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/00544-6 - Signaling components that travel between and transformed and nontransformed cells by microvesicles |
| Grantee: | Vanessa Morais Freitas |
| Support Opportunities: | Research Grants - Visiting Researcher Grant - International |
| FAPESP's process: | 10/07699-1 - Protease ADAMTS1 influencing breast cancer behavior and microenvironment |
| Grantee: | Vanessa Morais Freitas |
| Support Opportunities: | Research Grants - Young Investigators Grants |