Low-Level Laser Therapy (780 nm) on VEGF Modulation at Partially Injured Achilles Tendon

Author(s)

de Jesus, Julio Fernandes ; Spadacci-Morena, Diva Denelle ; dos Anjos Rabelo, Nayra Deise ; Pinfildi, Carlos Eduardo ; Fukuda, Thiago Yukio ; Plapler, Helio

• Document type: Journal article
• Source: Photomedicine and Laser Surgery; v. 34, n. 8, p. 331-335, AUG 2016.
• Web of Science Citations: 0

Abstract

Objective: The aim of this study was to analyze the modulatory effects of near infrared (780 nm) low-level laser therapy (LLLT) on the presence of the vascular endothelial growth factor (VEGF) in the partially injured Achilles tendons of rats. Background: LLLT stimulates the healing process for Achilles tendon injuries, although the extent of the modulatory effect of LLLT on the VEGF levels found in the injured tendons remains unclear. Methods: Sixty-five male Wistar rats were distributed in the following seven groups: LASER 1, 3, and 7 (10 partially injured Achilles tendons in each group, which were treated with LLLT for 1, 3, and 7 days, respectively); Sham 1, 3, and 7 (same injury, with simulated LLLT); Control group containing the five remaining animals and in which no procedures were performed. LLLT was applied once a day for 10 sec, with a mean power of 70mW and fluency of 17.5 J/cm(2). After euthanasia, all of the Achilles tendons were surgically removed and the VEGF levels were analyzed using immunohistochemistry. Results: The VEGF levels remained close to normal (p > 0.05) when comparing the experimental groups (LASER and Sham: 1, 3, and 7) with the Control group. Conclusion: LLLT did not stimulate the expression of VEGF in the treated Achilles tendons. (AU)

• FAPESP's process: 11/00979-1 - Low level laser influence in the modulation of inflammation and tissue repair of rat Achilles tendon after partial injury
• Grantee: Helio Plapler
• Support Opportunities: Regular Research Grants