| Full text | |
| Author(s): Show less - |
Melo, Igor S.
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Santos, Yngrid M. O.
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Costa, Maisa A.
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Pacheco, Amanda L. D.
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Silva, Nivea K. G. T.
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Cardoso-Sousa, L.
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Pereira, U. P.
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Goulart, L. R.
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Garcia-Cairasco, Norberto
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Duzzioni, Marcelo
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Gitai, Daniel L. G.
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Tilelli, Cristiane Q.
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Sabino-Silva, Robinson
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Castro, Olagide W.
Total Authors: 14
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| Document type: | Journal article |
| Source: | Epilepsy & Behavior; v. 61, p. 258-268, AUG 2016. |
| Web of Science Citations: | 4 |
| Abstract | |
Temporal lobe epilepsy (TLE) is characterized by spontaneous recurrent seizures, starting from secondary functional disorders due to several insults, including self-sustaining continuous seizures identified as status epilepticus (SE). Although hypoglycemia has been associated with SE, the effect of inhibition of the Na+/glucose cotransporters (SGLTs) on hippocampus during SE is still unknown. Here we evaluated the functional role of SGLT in the pattern of limbic seizures and neurodegeneration process after pilocarpine (PILO)-induced SE. Vehicle (VEH, 1 mu L) or phlorizin, a specific SGLT inhibitor (PZN, 1 mu L, 50 mu g/mu L), was administered in the hippocampus of rats 30 min before PILO (VEH + PILO or PZN + PILO, respectively). The limbic seizures were classified using the Racine's scale, and the amount of wet dog shakes (WDS) was quantified before and during SE. Neurodegeneration process was evaluated by Fluoro-Jade C (FJ-C), and FJ-C-positive neurons (FJ-C+) were counted 24 h and 15 days after SE. The PZN-treated rats showed higher (p < 0.05) number of WDS when compared with VEH + PILO. There was no difference in seizure severity between PZN + PILO and VEH + PILO groups. However, the pattern of limbic seizures significantly changed in PZN + PILO. Indeed, the class 5 seizures repeated themselves more times (p < 0.05) than the other classes in the PZN group at 50 min after SE induction. The PZN + PILO animals had a higher (p < 0.05) number of FJ-C + cells in the dentate gyrus (DG), hilus, and CA3 and CA1 of hippocampus, when compared with VEH + PILO. The PZN + PILO animals had a decreased number (p < 0.05) of FJ-C+ cells in CA1 compared with VEH + PILO 15 days after SE induction. Taken together, our data suggest that SGLT inhibition with PZN increased the severity of limbic seizures during SE and increased neurodegeneration in hippocampus 24 h after SE, suggesting that SGLT1 and SGLT2 could participate in the modulation of earlier stages of epileptogenic processes. (C) 2016 Elsevier Inc. All rights reserved. (AU) | |