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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunomodulatory/inflammatory effects of geopropolis produced by Melipona fasciculata Smith in combination with doxorubicin on THP-1 cells

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Author(s):
Garcia Oliveira, Lucas Pires ; Conte, Fernanda Lopes ; Cardoso, Eliza de Oliveira ; Conti, Bruno Jose ; Santiago, Karina Basso ; Golim, Marjorie de Assis ; Cruz, Maria Teresa ; Sforcin, Jose Mauricio
Total Authors: 8
Document type: Journal article
Source: Journal of Pharmacy and Pharmacology; v. 68, n. 12, p. 1551-1558, DEC 2016.
Web of Science Citations: 2
Abstract

ObjectivesGeopropolis (GEO) in combination with doxorubicin (DOX) reduced HEp-2 cells viability compared to GEO and DOX alone. A possible effect of this combination on the innate immunity could take place, and its effects were analysed on THP-1 cell - a human leukaemia monocytic cell line used as a model to study monocyte activity and macrophage activity, assessing cell viability, expression of cell markers and cytokine production. MethodsTHP-1 cells were incubated with GEO, DOX and their combination. Cell viability was assessed by MTT assay, cell markers expression by flow cytometry and cytokine production by ELISA. Key findingsGEO + DOX did not affect cell viability. GEO alone or in combination increased TLR-4 and CD80 but not HLA-DR and TLR-2 expression. GEO stimulated TNF- production while DOX alone or in combination did not affect it. GEO alone or in combination inhibited IL-6 production. ConclusionsGEO exerted a pro-inflammatory profile by increasing TLR-4 and CD80 expression and TNF- production, favouring the activation of the immune/inflammatory response. GEO + DOX did not affect cell viability and presented an immunomodulatory action. Lower concentrations of DOX combined to GEO could be used in cancer patients, avoiding side effects and benefiting from the biological properties of GEO. (AU)

FAPESP's process: 15/02638-8 - Possible imunotoxicological action of geopropolis associated with chemotherapy on human monocytes
Grantee:Lucas Pires Garcia Oliveira
Support Opportunities: Scholarships in Brazil - Scientific Initiation