Manool, a Salvia officinalis diterpene, induces selective cytotoxicity in cancer cells

Author(s)

de Oliveira, Pollyanna Francielli ; Munari, Carla Carolina ; Nicolella, Heloiza Diniz ; Sola Veneziani, Rodrigo Cassio ; Tavares, Denise Crispim

• Document type: Journal article
• Source: Cytotechnology; v. 68, n. 5, p. 2139-2143, OCT 2016.
• Web of Science Citations: 3

Abstract

Manool, a diterpene isolated from Salvia officinalis, was evaluated by the XTT colorimetric assay for cytotoxicity and selectivity against different cancer cell lines: B16F10 (murine melanoma), MCF-7 (human breast adenocarcinoma), HeLa (human cervical adenocarcinoma), HepG2 (human hepatocellular carcinoma), and MO59J, U343 and U251 (human glioblastoma). A normal cell line (V79, Chinese hamster lung fibroblasts) was used to compare the selectivity of the test substance. Manool exhibited higher cytotoxic activity against HeLa (IC50 = 6.7 +/- 1.1 A mu g/mL) and U343 (IC50 = 6.7 +/- 1.2 A mu g/mL) cells. In addition, in the used experimental protocols, the treatment with manool was significantly more cytotoxic for different tumor cell lines than for the normal cell line V79 (IC50 = 49.3 +/- 3.3 A mu g/mL), and showed high selectivity. These results suggest that manool may be used to treat cancer without affecting normal cells. (AU)

• FAPESP's process: 11/21310-2 - Study of cytotoxic, genotoxic and antioxidant activities of Styrax camporum hydroalcoholic extract and chemical markers, egonol and homoegonol, and their influence on genetic material damage and pre-neoplastic lesions.
• Grantee: Pollyanna Francielli de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate