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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Structural characterization of the major ampullate silk spidroin-2 protein produced by the spider Nephila clavipes

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Author(s):
Aparecido dos Santos-Pinto, Jose Roberto ; Arcuri, Helen Andrade ; Lubec, Gert ; Palma, Mario Sergio
Total Authors: 4
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1864, n. 10, p. 1444-1454, OCT 2016.
Web of Science Citations: 3
Abstract

Major ampullate spidroin-2 (MaSp2) is one of the most important spider silk protein, but up to now no information is available regarding the post-translational modifications (PTMs) of this protein. A gel-based mass spectrometry strategy using collision-induced dissociation (CID) and electron-transfer dissociation (ETD) fragmentation methods was used to sequence Nephila clavipes MaSp2 (including the N- and C-terminal non repetitive domains, and the great part of the central core), and to assign a series of post-translational modifications (PTMs) on to the MaSp2 sequence. Two forms of this protein were identified, with different levels of phosphorylation along their sequences. These findings provide a basis for understanding mechanoelastic properties and can support the future design of recombinant spider silk proteins for biotechnological applications. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/19051-6 - Structural analysis of silk proteins of Nephila clavipes spider web by a proteomic approach.
Grantee:José Roberto Aparecido dos Santos-Pinto
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/26451-9 - Bioprospecting and Structural Analysis of the Silk Proteins of Arthropods by a Proteomics Approach Using nanoLC-ESI-CID/ETD System
Grantee:José Roberto Aparecido dos Santos-Pinto
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/51684-1 - System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants