| Full text | |
| Author(s): |
Rustiguel, Joane K.
;
Soares, Ricardo O. S.
;
Meisburger, Steve P.
;
Davis, Katherine M.
;
Malzbender, Kristina L.
;
Ando, Nozomi
;
Dias-Baruffi, Marcelo
;
Nonato, Maria Cristina
Total Authors: 8
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 6, SEP 19 2016. |
| Web of Science Citations: | 3 |
| Abstract | |
Galectins are proteins involved in diverse cellular contexts due to their capacity to decipher and respond to the information encoded by beta-galactoside sugars. In particular, human galectin-4, normally expressed in the healthy gastrointestinal tract, displays differential expression in cancerous tissues and is considered a potential drug target for liver and lung cancer. Galectin-4 is a tandem-repeat galectin characterized by two carbohydrate recognition domains connected by a linker-peptide. Despite their relevance to cell function and pathogenesis, structural characterization of full-length tandem-repeat galectins has remained elusive. Here, we investigate galectin-4 using X-ray crystallography, small-and wide-angle X-ray scattering, molecular modelling, molecular dynamics simulations, and differential scanning fluorimetry assays and describe for the first time a structural model for human galectin-4. Our results provide insight into the structural role of the linker-peptide and shed light on the dynamic characteristics of the mechanism of carbohydrate recognition among tandem-repeat galectins. (AU) | |
| FAPESP's process: | 11/21811-1 - Structural characterization and evaluation of funciontal aspects of tanden-repeat human galectins |
| Grantee: | Maria Cristina Nonato |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/16153-2 - Structural characterization and evaluation of functional aspects of human galectins from tandem-repeat group |
| Grantee: | Joane Kathelen Rustiguel Bonalumi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |