Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua

Author(s)

Sato, Ana Claudia ; Bosch, Rosemary Viola ; Alves Will, Sonia Elisabete ; Alvarez-Flores, Miryam Paola ; Goldfeder, Mauricio Barbugiani ; Mesquita Pasqualoto, Kerly Fernanda ; Vaz Galvao da Silva, Brbara Athayde ; de Andrade, Sonia Aparecida ; Chudzinski-Tavassi, Ana Marisa

• Document type: Journal article
• Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 22, DEC 21 2016.
• Web of Science Citations: 1

Abstract

Abstract Background Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats. Methods Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the following tissue regeneration parameters were evaluated: wound closure and collagen content. Furthermore, tissue sections were subjected to histological and immunohistochemical analyses. Results The rLosac treatment has demonstrated its capacity to improve wound healing, as reflected in findings of a larger number of activated fibroblasts, proliferation of epithelial cells, increase of collagen type 1, and decrease of inflammatory infiltrate. Conclusion The findings have indicated the rLosac protein as a very promising molecule for the development of new wound-healing formulations. (AU)

• FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
• Grantee: Hugo Aguirre Armelin
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/06892-0 - Multifunctional molecules from Lonomia obliqua caterpillar: designing of new chemical entities and interrelationship with cellular mechanisms
• Grantee: Ana Marisa Chudzinski-Tavassi
• Support Opportunities: Regular Research Grants