Development of lipid nanodispersions containing insulin for the topical treatment ...
| Full text | |
| Author(s): |
Ogunjimi, Abayomi T.
;
Melo, Shaiani M. G.
;
Vargas-Rechia, Carem G.
;
Emery, Flavio S.
;
Lopez, Renata F. V.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | Carbohydrate Polymers; v. 157, p. 1065-1075, FEB 10 2017. |
| Web of Science Citations: | 12 |
| Abstract | |
Delonix is a galactomannan polysaccharide extracted from the endosperm of Delonix regia plant. This study aims at the development of Delonix nanoparticle and assesses its potential for ocular delivery by evaluating its in-vitro stability, toxicity and cellular uptake. Fluorescent nanoparticles (BODIPY-loaded nanoparticles) were prepared by a Quality-by-Design modified nanoprecipitation technique. Optimized nanoparticles had mean sizes <240 nm, PdI <0.2 and zeta potential of <-30 mV. Mixture of surfactants with different hydrophilic-lipophilic balance controlled nanoparticle swelling. Nanoparticles, which were stable in the presence of simulated lachrymal fluid and lysozyme also sustained the release of BODIPY. In-vitro studies suggest no toxicity of the nanoparticles in concentration range of 100-1483.3 mu g/mL on retinal and corneal epithelial cells. Flow cytometry and confocal microscopy techniques showed that retinal cells but not corneal cells, uptake 18% of the nanoparticles. Therefore, Delonix nanoparticles could be a safe and promising tool for ocular drug delivery. (C) 2016 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue |
| Grantee: | Renata Fonseca Vianna Lopez |
| Support type: | Research Projects - Thematic Grants |