| Full text | |
| Author(s): |
Collaco, Rita de Cassia O.
;
Randazzo-Moura, Priscila
;
Tamascia, Mariana L.
;
da Silva, Igor Rapp F.
;
Rocha, Thalita
;
Cogo, Jose C.
;
Hyslop, Stephen
;
Sanny, Charles G.
;
Rodrigues-Simioni, Lea
Total Authors: 9
|
| Document type: | Journal article |
| Source: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 191, p. 86-100, JAN 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
In this work, we examined some biochemical and biological activities of Bothrops fonsecai venom, a pitviper endemic to southeastern Brazil, and assessed their neutralization by commercial bothropic antivenom (CAv). Cross-reactivity of venom with CAv was also assessed by immunoblotting and size-exclusion high performance chromatography (SE-HPLC). Bothrops fonsecai venom had PLA(2), proteolytic and esterase activities that were neutralized to varying extents by venom:antivenom ratios of 5:1 and 5:2 (PLA2 and esterase activities) or not significantly by either venom:antivenom ratio (proteolytic activity). The minimum hemorrhagic dose (69.2 mu g) was totally neutralized by both ratios. Clotting time in rat citrated plasma was 33 +/- 10.5 s (mean +/- SD; n = 5) and was completely neutralized by a 5:2 ratio. Edema formation was dose-dependent (1-30 mu g/site) and significantly inhibited by both ratios. Venom (10-300 mu g/mL) caused neuromuscular blockade in extensor digitorum longus preparations; this blockade was inhibited best by a 5:2 ratio. Venom caused myonecrosis and creatine kinase release in vivo (gastrocnemius muscle) and in vitro (extensor digitorum longus) that was effectively neutralized by both venom:antivenom ratios. Immunoblotting showed that venom components of similar to 25-100 kDa interacted with CAv. SE-HPLC profiles for venom incubated with CAv or specific anti-B. fonsecai antivenom raised in rabbits (SAv) indicated that CAv had a higher binding capacity than SAv, whereas SAv had higher affinity than CAv. These findings indicate that B. fonsecai venom contains various activities that are neutralized to different extents by CAv and suggest that CAv could be used to treat envenoming by B. fonsecai. (C) 2016 Published by Elsevier Inc. (AU) | |
| FAPESP's process: | 12/06165-9 - Study of biological activities induced by Bothrops fonsecai snake venom and the uso of commercial bothropic antivenom as a pharmacological tool. |
| Grantee: | Rita de Cássia de Oliveira Collaço |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/24444-2 - Analysis of antibody-antigen interactions (venom: antivenom) using size-exclusion high-performance liquid chromatography |
| Grantee: | Rita de Cássia de Oliveira Collaço |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |