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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation

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Author(s):
Teixeira, Lucas Novaes ; Spinola de Castro Raucci, Larissa Moreira ; Alonso, Gabriela Caroline ; Della Coletta, Ricardo ; Rosa, Adalberto Luiz ; de Oliveira, Paulo Tambasco
Total Authors: 6
Document type: Journal article
Source: TUMOR BIOLOGY; v. 37, n. 9, p. 12371-12385, SEP 2016.
Web of Science Citations: 3
Abstract

This study evaluated the temporal expression of osteopontin (OPN) in co-cultures of human osteoblastic cells (SAOS-2) and oral squamous cell carcinoma (OSCC)-derived cells (SCC9) and examined the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of these co-cultures on subsequent osteoclastic activity were explored. SCC9 cells were plated on TranswellA (R) membranes that were either coated or not coated with Matrigel and were then co-cultured with SAOS-2 cells during the peak of OPN expression. SCC9 cells exposed to OPN-silenced SAOS-2 cultures and SCC9 cells cultured alone served as controls. SCC9 cells were quantitatively evaluated for cell adhesion, proliferation, migration, and invasion into Matrigel. The impact of co-culturing SAOS-2 and SCC9 cells on the resorptive capacity of U-937-derived osteoclastic cells was also investigated. Furthermore, a reciprocal induction of SAOS-2 and SCC9 cells in terms of OPN expression over the co-culture interval was identified. SAOS-2-secreted OPN altered the SCC9 cell phenotype, leading to enhanced cell adhesion and proliferation and higher Matrigel invasion. This invasion was also enhanced, albeit to a lesser degree, by co-culture with OPN-silenced SAOS-2 cells. Cell migration was not affected. Co-culture with SAOS-2 cells-mainly during the period of peak OPN expression-promoted over-expression of IL-6 and IL-8 by SCC9 cells and enhanced the resorptive capacity of osteoclastic cells. Taken together, these results suggest that osteoblast-derived OPN affects the interactions among OSCC-derived epithelial cells, osteoblasts, and osteoclasts, which could contribute to the process of bone destruction during bone invasion by OSCC. (AU)

FAPESP's process: 12/20863-0 - Expression of osteopontin in cocultures of osteoblastic cells and squamous cell carcinoma cells and its effects on the neoplastic cell phenotype and osteoclastic activation
Grantee:Gabriela Caroline Alonso
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/07531-9 - Expression of osteopontin in cocultures of osteoblastic cells and squamous cell carcinoma cells and its effects on the neoplastic cell phenotype and osteoclastic activation
Grantee:Paulo Tambasco de Oliveira
Support type: Regular Research Grants
FAPESP's process: 12/08605-6 - Expression of osteopontin in cocultures of osteoblastic cells and squamous cell carcinoma cells and its effects on the neoplastic cell phenotype and osteoclastic activation
Grantee:Lucas Novaes Teixeira
Support type: Scholarships in Brazil - Doctorate