Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation

Author(s)

Teixeira, Lucas Novaes ; Spinola de Castro Raucci, Larissa Moreira ; Alonso, Gabriela Caroline ; Della Coletta, Ricardo ; Rosa, Adalberto Luiz ; de Oliveira, Paulo Tambasco

• Document type: Journal article
• Source: TUMOR BIOLOGY; v. 37, n. 9, p. 12371-12385, SEP 2016.
• Web of Science Citations: 3

Abstract

This study evaluated the temporal expression of osteopontin (OPN) in co-cultures of human osteoblastic cells (SAOS-2) and oral squamous cell carcinoma (OSCC)-derived cells (SCC9) and examined the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of these co-cultures on subsequent osteoclastic activity were explored. SCC9 cells were plated on TranswellA (R) membranes that were either coated or not coated with Matrigel and were then co-cultured with SAOS-2 cells during the peak of OPN expression. SCC9 cells exposed to OPN-silenced SAOS-2 cultures and SCC9 cells cultured alone served as controls. SCC9 cells were quantitatively evaluated for cell adhesion, proliferation, migration, and invasion into Matrigel. The impact of co-culturing SAOS-2 and SCC9 cells on the resorptive capacity of U-937-derived osteoclastic cells was also investigated. Furthermore, a reciprocal induction of SAOS-2 and SCC9 cells in terms of OPN expression over the co-culture interval was identified. SAOS-2-secreted OPN altered the SCC9 cell phenotype, leading to enhanced cell adhesion and proliferation and higher Matrigel invasion. This invasion was also enhanced, albeit to a lesser degree, by co-culture with OPN-silenced SAOS-2 cells. Cell migration was not affected. Co-culture with SAOS-2 cells-mainly during the period of peak OPN expression-promoted over-expression of IL-6 and IL-8 by SCC9 cells and enhanced the resorptive capacity of osteoclastic cells. Taken together, these results suggest that osteoblast-derived OPN affects the interactions among OSCC-derived epithelial cells, osteoblasts, and osteoclasts, which could contribute to the process of bone destruction during bone invasion by OSCC. (AU)

• FAPESP's process: 12/20863-0 - Expression of osteopontin in cocultures of osteoblastic cells and squamous cell carcinoma cells and its effects on the neoplastic cell phenotype and osteoclastic activation
• Grantee: Gabriela Caroline Alonso
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/07531-9 - Expression of osteopontin in cocultures of osteoblastic cells and squamous cell carcinoma cells and its effects on the neoplastic cell phenotype and osteoclastic activation
• Grantee: Paulo Tambasco de Oliveira
• Support type: Regular Research Grants
• FAPESP's process: 12/08605-6 - Expression of osteopontin in cocultures of osteoblastic cells and squamous cell carcinoma cells and its effects on the neoplastic cell phenotype and osteoclastic activation
• Grantee: Lucas Novaes Teixeira
• Support type: Scholarships in Brazil - Doctorate