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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle

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Author(s):
da Silva Diniz, Wellison Jarles ; Coutinho, Luiz Lehmann ; Tizioto, Polyana Cristine ; Mello Cesar, Aline Silva ; Gromboni, Caio Fernando ; Araujo Nogueira, Ana Rita ; Neubern de Oliveira, Priscila Silva ; de Souza, Marcela Maria ; de Almeida Regitano, Luciana Correia
Total Authors: 9
Document type: Journal article
Source: PLoS One; v. 11, n. 8 AUG 17 2016.
Web of Science Citations: 5
Abstract

Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter. Eight Nelore steers, with extreme genomic estimated breeding values for iron content (Fe-GEBV), were selected from a reference population of 373 animals. From the 49 annotated DE genes (FDR<0.05) found between the two groups, 18 were up-regulated and 31 down-regulated for the animals in the low Fe-GEBV group. The functional enrichment analyses identified several biological processes, such as lipid transport and metabolism, and cell growth. Lipid metabolism was the main pathway observed in the analysis of metabolic and canonical signaling pathways for the genes identified as DE, including the genes FASN, FABP4, and THRSP, which are functional candidates for beef quality, suggesting reduced lipogenic activities with lower iron content. Our results indicate metabolic pathways that are partially influenced by iron, contributing to a better understanding of its participation in skeletal muscle physiology. (AU)

FAPESP's process: 12/23638-8 - Molecular basis of meat quality in Nelore beef cattle
Grantee:Luciana Correia de Almeida Regitano
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/12652-5 - Differential gene expression related to iron content in Nellore muscle
Grantee:Wellison Jarles da Silva Diniz
Support Opportunities: Scholarships in Brazil - Master