In vivo confocal Raman spectroscopy and molecular dynamics analysis of penetration of retinyl acetate into stratum corneum

Author(s)

dos Santos, Laurita ; Tellez S, Claudio A. ; Sousa, Mariane P. J. ; Azoia, Nuno G. ; Cavaco-Paulo, Artur M. ; Martin, Airton A. ; Favero, Priscila P.

• Document type: Journal article
• Source: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 174, p. 279-285, MAR 5 2017.
• Web of Science Citations: 7

Abstract

Objective: The purpose of this study is to elucidate the behavior of retinyl acetate in penetrating human skin without the presence of enhancers by using confocal Raman spectroscopy and molecular dynamics simulation. Methods: In this study, in vivo confocal Raman spectroscopy was combined with molecular dynamics simulation to investigate the transdermal permeation of the aqueous suspension of retinyl acetate. Results: Permeation,was measured after 30 min, and retinyl acetate was found up to 20 pm deep inside the stratum corneum. The delivery of retinyl acetate inside a skin membrane model was studied by molecular dynamics. The membrane model that was used represented normal young skin containing a lipid bilayer with 25% ceramide, 36% fatty add, 30% cholesterol, and 6% cholesterol sulfate. Conclusion: Spectroscopy data indicate that retinyl acetate permeates into the stratum corneum. Molecular dynamics data showed that retinyl acetate permeates in the membrane model and that their final location is deep inside the lipid bilayer. We showed, for the first time, a correlation between Raman permeation data and computational data. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/05975-2 - Cosmetic permeation through human skin: theoretical and computational analysis
• Grantee: Airton Abrahao Martin
• Support Opportunities: Research Grants - Visiting Researcher Grant - International