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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil

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Author(s):
Eloiza Helena Campana [1] ; Danilo Elias Xavier [2] ; Fernanda Villas-Boas Petrolini [3] ; Jhonatha Rodrigo Cordeiro-Moura [4] ; Maria Rita Elmor de Araujo [5] ; Ana Cristina Gales [6]
Total Authors: 6
Affiliation:
[1] Universidade Federal de São Paulo. Laboratório ALERTA. Disciplina de Infectologia - Brasil
[2] Universidade Federal de São Paulo. Laboratório ALERTA. Disciplina de Infectologia - Brasil
[3] Universidade Federal de São Paulo. Laboratório ALERTA. Disciplina de Infectologia - Brasil
[4] Universidade Federal de São Paulo. Laboratório ALERTA. Disciplina de Infectologia - Brasil
[5] Hospital Benemérita Sociedade Portuguesa de Beneficência - Brasil
[6] Universidade Federal de São Paulo. Laboratório ALERTA. Disciplina de Infectologia - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Journal of Infectious Diseases; v. 21, n. 1, p. 57-62, 2017-02-00.
Abstract

Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates. (AU)

FAPESP's process: 10/12891-9 - Evaluation of Pseudomonas aeruginosa, Acinetobacter Baumannii and Klebsiella pneumoniae transcriptomes for elucidation of their polymyxin resistance mechanisms
Grantee:Ana Cristina Gales
Support Opportunities: Regular Research Grants