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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The PacC transcription factor regulates secondary metabolite production and stress response, but has only minor effects on virulence in the insect pathogenic fungus Beauveria bassiana

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Author(s):
Luo, Zhibing ; Ren, Hui ; Mousa, Jarrod J. ; Rangel, Drauzio E. N. ; Zhang, Yongjun ; Bruner, Steven D. ; Keyhani, Nemat O.
Total Authors: 7
Document type: Journal article
Source: ENVIRONMENTAL MICROBIOLOGY; v. 19, n. 2, p. 788-802, FEB 2017.
Web of Science Citations: 12
Abstract

The PacC transcription factor is an important component of the fungal ambient pH-responsive regulatory system. Loss of pacC in the insect pathogenic fungus Beauveria bassiana resulted in an alkaline pH-dependent decrease in growth and pH-dependent increased susceptibility to osmotic (salt, sorbitol) stress and SDS. Extreme susceptibility to Congo Red was noted irrespective of pH, and BbpacC conidia showed subtle increases in UV susceptibility. The BbPacC mutant showed a reduced ability to acidify media during growth due to failure to produce oxalic acid. The BbPacC mutant also did not produce the insecticidal compound dipicolinic acid, however, production of a yellow-colored compound was noted. The compound, named bassianolone B, was purified and its structure determined. Despite defects in growth, stress resistance, and oxalate/insecticidal compound production, only a small decrease in virulence was seen for the BbpacC strain in topical insect bioassays using larvae from the greater waxmoth, Galleria mellonella or adults of the beetle, Tenebrio molitor. However, slightly more pronounced decreases were seen in virulence via intrahemcoel injection assays (G. mellonella) and in assays using T. molitor larvae. These data suggest important roles for BbpacC in mediating growth at alkaline pH, regulating secondary metabolite production, and in targeting specific insect stages. (AU)

FAPESP's process: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi
Grantee:Drauzio Eduardo Naretto Rangel
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions
Grantee:Drauzio Eduardo Naretto Rangel
Support Opportunities: Regular Research Grants