Comparative functional studies of the activator of Hsp90 ATPase 1 (AHA1) of Leishm...
| Full text | |
| Author(s): |
Seraphim, Thiago V.
;
Silva, Kelly P.
;
Dores-Silva, Paulo R.
;
Barbosa, Leandro R. S.
;
Borges, Julio C.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 97, p. 503-512, APR 2017. |
| Web of Science Citations: | 6 |
| Abstract | |
Heat shock protein of 90 kDa (Hsp90) is an essential molecular chaperone involved in a plethora of cellular activities which modulate protein homeostasis. During the Hsp90 mechanochemical cycle, it undergoes large conformational changes, oscillating between open and closed states. Although structural and conformational equilibria of prokaryotic and some eukaryotic Hsp90s are known, some protozoa Hsp90 structures and dynamics are poorly understood. In this study, we report the solution structure and conformational dynamics of Leishmania braziliensis Hsp90 (LbHsp90) investigated by small angle X-ray scattering (SAXS). The results indicate that LbHsp90 coexists in open and closed conformations in solution and that the linkers between domains are not randomly distributed. These findings noted interesting features of the LbHsp90 system, opening doors for further conformational studies of other protozoa chaperones. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 14/07206-6 - Studies of the mitochondrial HSP70 of human and protozoa: structural and functional approaches |
| Grantee: | Julio Cesar Borges |
| Support type: | Regular Research Grants |
| FAPESP's process: | 12/50161-8 - Study of the structure and function of the Hsp90 chaperone with emphasis on its role in cellular homeostasis |
| Grantee: | Carlos Henrique Inacio Ramos |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 11/23110-0 - Using isothermal titration calorimetry for the determination of thermodynamic properties of protein-ligand and protein-protein interactions |
| Grantee: | Julio Cesar Borges |
| Support type: | Regular Research Grants |