Giant FAZ10 is required for flagellum attachment zone stabilization and furrow positioning in Trypanosoma brucei

Author(s)

Moreira, Bernardo P. ; Fonseca, Carol K. ; Hammarton, Tansy C. ; Baqui, Munira M. A.

• Document type: Journal article
• Source: Journal of Cell Science; v. 130, n. 6, p. 1179-1193, MAR 15 2017.
• Web of Science Citations: 7

Abstract

The flagellum and flagellum attachment zone (FAZ) are important cytoskeletal structures in trypanosomatids, being required for motility, cell division and cell morphogenesis. Trypanosomatid cytoskeletons contain abundant high molecular mass proteins (HMMPs), but many of their biological functions are still unclear. Here, we report the characterization of the giant FAZ protein, FAZ10, in Trypanosoma brucei, which, using immunoelectron microscopy, we show localizes to the intermembrane staples in the FAZ intracellular domain. Our data show that FAZ10 is a giant cytoskeletal protein essential for normal growth and morphology in both procyclic and bloodstream parasite life cycle stages, with its depletion leading to defects in cell morphogenesis, flagellum attachment, and kinetoplast and nucleus positioning. We show that the flagellum attachment defects are probably brought about by reduced tethering of the proximal domain of the paraflagellar rod to the FAZ filament. Further, FAZ10 depletion also reduces abundance of FAZ flagellum domain protein, ClpGM6. Moreover, ablation of FAZ10 impaired the timing and placement of the cleavage furrow during cytokinesis, resulting in premature or asymmetrical cell division. (AU)

• FAPESP's process: 10/19547-1 - Functional characterization of trypanosomatid giant proteins
• Grantee: Munira Muhammad Abdel Baqui
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/22129-2 - Functional characterization of giant protein in Trypanosoma brucei
• Grantee: Bernardo Pereira Moreira
• Support Opportunities: Scholarships in Brazil - Doctorate