Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The repetitive DNA element BncDNA, enriched in the B chromosome of the cichlid fish Astatotilapia latifasciata, transcribes a potentially noncoding RNA

Full text
Author(s):
Ramos, Erica ; Cardoso, Adauto L. ; Brown, Judith ; Marques, Diego F. ; Fantinatti, Bruno E. A. ; Cabral-de-Mello, Diogo C. ; Oliveira, Rogerio A. ; O'Neill, Rachel J. ; Martins, Cesar
Total Authors: 9
Document type: Journal article
Source: CHROMOSOMA; v. 126, n. 2, p. 313-323, MAR 2017.
Web of Science Citations: 15
Abstract

Supernumerary chromosomes have been studied in many species of eukaryotes, including the cichlid fish, Astatotilapia latifasciata. However, there are many unanswered questions about the maintenance, inheritance, and functional aspects of supernumerary chromosomes. The cichlid family has been highlighted as a model for evolutionary studies, including those that focus on mechanisms of chromosome evolution. Individuals of A. latifasciata are known to carry up to two B heterochromatic isochromosomes that are enriched in repetitive DNA and contain few intact gene sequences. We isolated and characterized a transcriptionally active repeated DNA, called B chromosome noncoding DNA (BncDNA), highly represented across all B chromosomes of A. latifasciata. BncDNA transcripts are differentially processed among six different tissues, including the production of smaller transcripts, indicating transcriptional variation may be linked to B chromosome presence and sexual phenotype. The transcript lengths and lack of similarity with known protein/gene sequences indicate BncRNA might represent a novel long noncoding RNA family (lncRNA). The potential for interaction between BncRNA and known miRNAs were computationally predicted, resulting in the identification of possible binding of this sequence in upregulated miRNAs related to the presence of B chromosomes. In conclusion, Bnc is a transcriptionally active repetitive DNA enriched in B chromosomes with potential action over B chromosome maintenance in somatic cells and meiotic drive in gametic cells. (AU)

FAPESP's process: 12/20959-8 - Structural and functional analysis of transcripts from non-coding DNA BncDNA associated with the presence of B chromosomes in Astatotilapia latifasciata
Grantee:Érica Ramos
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/17212-2 - Genomic organization of ribosomal DNA in the B chromosomes of Haplochromis obliquidens (Perciformes, Cichlidae)
Grantee:Érica Ramos
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/04533-3 - Functional analysis of B chromosomes: the cichlid fish Astatotilapia latifasciata as model
Grantee:César Martins
Support Opportunities: Regular Research Grants