Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma

Author(s)

Conceicao, Andre Luis Giacometti ; Da Silva, Camila Tainah ; Badial, Rodolfo Miglioli ; Valsechi, Marina Curado ; Stuqui, Bruna ; Goncalves, Jessica Domingues ; Jasiulionis, Miriam Galvonas ; Calmon, Marilia De Freitas ; Rahal, Paula

• Document type: Journal article
• Source: ONCOLOGY REPORTS; v. 37, n. 3, p. 1487-1496, MAR 2017.
• Web of Science Citations: 4

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. This carcinoma is histologically characterized by the presence of clear and abundant cytoplasm. In the present study, we sought to identify genes differentially expressed in ccRCC and build a molecular profile of this cancer. We selected genes described in the literature related to cellular differentiation and proliferation. We analyzed the gene and protein expression by quantitative PCR (qPCR) and immunohistochemistry, respectively, and examined possible epigenetic mechanisms that regulate their expression in ccRCC samples and cell lines. Occludin (OCLN) and growth arrest-specific 1 (GAS1) genes were underexpressed in ccRCC, and we report that miR-122 and miR-34a, respectively, may regulate their expression in this cancer. Furthermore, we showed by qPCR and immunohistochemistry that solute carrier family 2 member 1 (SLC2A1) was significantly overexpressed in ccRCC. The set of genes identified in the present study furthers our understanding of the molecular basis and development of ccRCC. (AU)

• FAPESP's process: 12/08853-0 - Analysis of the expression of genes involved in epithelium mesenchyme transition in renal clear cell carcinoma
• Grantee: Andre Luis Giacometti Conceicao
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)