Sleep influences the immune response and the rejection process alters sleep pattern: Evidence from a skin allograft model in mice

Author(s)

Ruiz, Francieli Silva ; Andersen, Monica Levy ; Guindalini, Camila ; Araujo, Leandro Pires ; Lopes, Jose Daniel ; Tufik, Sergio

• Document type: Journal article
• Source: BRAIN BEHAVIOR AND IMMUNITY; v. 61, p. 274-288, MAR 2017.
• Web of Science Citations: 5

Abstract

Introduction: Sleep generally regulates immune functions in a supportive manner and can affect parameters that are directly involved in the rejection process. Study objectives: The first objective was to assess whether sleep deprivation (SD) or sleep restriction (SR) affects the allograft rejection process in mice. The second objective was to investigate whether the rejection process itself modulates the sleep pattern of allografted mice. Design: Adult BALB/c and C57BL/6J male mice were used as the donors and recipients, respectively, except for the syngeneic group (ISOTX), which received skin from mice of the same strain (C57BL/6J). The recipients were randomly assigned to either one of two control groups - TX (allogenic) or ISOTX (syngeneic) - which underwent stereotaxic surgery to enable sleep recording prior to the allograft but were not sleep deprived; one of two paradoxical sleep deprived groups - SDTX and TXSD - which underwent 72 h of continuous SD either before or after the allograft respectively, and one of two sleep restricted groups - SRTX and TXSR - which underwent 21 h of SD and 3 h of sleep for 15 days either before or after the allograft respectively. Interventions: The skin allograft was inspected daily to determine the survival time, expected as 8.0 +/- 0.4 days in this transplant model under no treatment. The sleep pattern was controlled throughout the rejection process in the SD and SR groups. Draining lymph nodes, spleen, blood and skin grafts were harvested on the 5th day after transplantation for evaluation of the immune parameters related to allograft rejection. Measurements and results: In the control groups, we observed a reduction in paradoxical sleep throughout the entire allograft rejection process. Acute and chronic experimental sleep loss in the SD and SR groups produced marked alterations in the immune response. Both SD and SR prolonged allograft survival compared to the non-sleep-deprived group. There were reductions in the following parameters involved in the allograft rejection under sleep loss: CD4(+) and CD8(+) T cell subpopulations in the peripheral lymph organs and spleen, circulating sIL-2R levels, graft-infiltrating CD4(+) T cells and skin allograft global gene expression. Conclusions: We provide, as far as we are aware, the first evidence in vivo that the immune response can alter the normal sleep pattern, and that sleep loss can conversely affect the immune response related to graft rejection. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 07/55445-6 - Sleep and immunity: evidence in healthy volunteers and in animal model of skin transplantation
• Grantee: Francieli Ruiz da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 98/14303-3 - Center for Sleep Studies
• Grantee: Sergio Tufik
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC