Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phosphosite-specific regulation of the oxidative-stress response of Paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis

Full text
Author(s):
Chaves, Alison F. A. [1] ; Castilho, Daniele G. [1] ; Navarro, Marina V. [1] ; Oliveira, Ana K. ; Serrano, Solange M. T. [2] ; Tashima, Alexandre K. ; Batista, Wagner L.
Total Authors: 7
Affiliation:
[1] Inst Butantan, Ctr Toxins, Lab Appl Toxinol, Immune Response & Cell Signaling, Sao Paulo, SP, Brazil. Tashima, Alexandre K., Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil. Batista, Wagner L., Univ Fed Sao Paulo, Dept Pharmaceut Sci, Diadema, SP - Brazil
[2] Batista, Wagner L., Univ Fed Sao Paulo, Dept Microbiol Immunol \& Parasitol, Sao Paulo, SP, Brazil. Oliveira, Ana K., Inst Butantan, Ctr Toxins, Lab Appl Toxinol, Immune Response & Cell Signaling, Sao Paulo, SP, Brazil. Tashima, Alexandre K., Univ Fed Sao - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Microbes and Infection; v. 19, n. 1, p. 34-46, JAN 2017.
Web of Science Citations: 3
Abstract

Paracoccidioides brasiliensis, a thermally dimorphic fungus, is the causative agent of paracoccidioidomycosis, a systemic mycosis that is widespread in Latin America. This fungus is a facultative intracellular pathogen able to survive and replicate inside non-activated macrophages. Therefore, the survival of P. brasiliensis inside the host depends on the ability to adapt to oxidative stress induced by immune cells, especially alveolar macrophages. For several years, reactive oxygen species (ROS) were only associated with pathological processes. Currently, a plethora of roles for ROS in cell signaling have emerged. We have previously reported that low ROS concentrations cause cell proliferation in the human pathogenic fungus P. brasiliensis. In the present report, we investigated the influence of phosphorylation events in that process. Using a mass spectrometry-based approach, we mapped 440 phosphorylation sites in 230 P. brasiliensis proteins and showed that phosphorylation at different sites determines fungal responses to oxidative stress, which are regulated by phosphatases and kinases activities. Furthermore, we present additional evidence for a functional two-component signal transduction system in P. brasiliensis. These findings will help us to understand the phosphorylation events involved in the oxidative stress response. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 11/14392-2 - Evaluation of the involvement of Ras GTPase from Paracoccidioides brasiliensis in thermo-dimorphism and during oxidative and nitrosative stress
Grantee:Wagner Luiz Batista
Support type: Regular Research Grants
FAPESP's process: 14/13961-1 - Role of secreted aspartyl protease (PbSAP) in virulence and dimorphism in the pathogenic fungus Paracoccidioides brasiliensis
Grantee:Wagner Luiz Batista
Support type: Regular Research Grants
FAPESP's process: 12/19321-9 - Proteomic and peptidomic Characterization of Brazilian spider venoms by mass spectrometry
Grantee:Alexandre Keiji Tashima
Support type: Regular Research Grants
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/17530-0 - Evaluation of Paracoccidioides brasiliensis phosphoproteome under oxidative stress
Grantee:Alison Felipe Alencar Chaves
Support type: Scholarships in Brazil - Master