Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes

Author(s)

Luevano-Martinez, Luis Alberto ; Forni, Maria Fernanda ; Peloggia, Julia ; Watanabe, Ii-Sei ; Kowaltowski, Alicia J.

• Document type: Journal article
• Source: MECHANISMS OF AGEING AND DEVELOPMENT; v. 162, p. 9-17, MAR 2017.
• Web of Science Citations: 8

Abstract

Calorie restriction (CR) has been amply demonstrated to modify mitochondrial function. However, little is known regarding the effects of this dietary regimen on mitochondrial membranes. We isolated phospholipids from rat liver mitochondria from animals on CR or ad libitum diets and found that mitochondria from ad libitum animals present an increased content of lipoperoxides and the content of cardiolipin. Cardiolipin is the main anionic phospholipid present in mitochondrial membranes, and plays a key role in mitochondria( function, signaling and stress response. Expression levels of the enzymes involved in cardiolipin biosynthesis and remodeling were quantified and found to be upregulated in CR animals. Interestingly, when mitochondria( membranes were fractionated, the outer membrane presented a higher content of cardiolipin, indicating a redistribution of this phospholipid mediated by a phospholipid scram blase in CR. This change is associated with Drp1-mediated mitochondrial fragmentation and autophagy. Overall, we find that CR promotes extensive mitochondrial membrane remodeling, decreasing oxidatively damaged lipids, and increasing cardiolipin levels and redistributing cardiolipin. These changes in membrane properties are consistent with and may be causative of changes in mitochondrial morphology, function and turnover previously found to occur in CR. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
• Grantee: Ohara Augusto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/04871-6 - Effects of caloric restriction on mitochondrial morphology, dynamics, bioenergetics and redox state
• Grantee: Maria Fernanda Pereira de Araújo Demonte Forni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/04919-9 - Evaluation of the adaptive response of mitochondrial membranes to stress: role of cardiolipin in membrane plasticity, dynamics and pathological situations
• Grantee: Luis Alberto Luevano Martinez
• Support Opportunities: Scholarships in Brazil - Post-Doctoral