Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition

Author(s):Show less -

Cardoso, Vinicius Saura ; Filgueiras, Marcelo de Carvalho ; Dutra, Yago Medeiros ; Gomes Teles, Ramon Handerson ; de Araujo, Alyne Rodrigues ; Primo, Fernando Lucas ; Mafud, Ana Carolina ; Batista, Larissa Fernandes ; Mascarenhas, Yvonne Primerano ; Martinez Paino, Ieda Maria ; Zucolotto, Valtencir ; Tedesco, Antonio Claudio ; Silva, Durcilene Alves ; Leite, Jose Roberto S. A. ; dos Santos, Jr., Jose Ribeiro

• Document type: Review article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 74, p. 382-388, MAY 1 2017.
• Web of Science Citations: 3

Abstract

Collagen is considered the most abundant protein in the animal kingdom, comprising 30% of the total amount of proteins and 6% of the human body by weight Studies that examine the interaction between silver nanoparticles and proteins have been highlighted in the literature in order to understand the stability of the nanoparticle system, the effects observed in biological systems, and the appearance of new chemical pharmaceutical products. The objective of this study was to analyze the behavior of silver nanoparticles stabilized with collagen (AgNPcol) and to check the skin permeation capacity and action in paw edema induced by carrageenan. AgNPcol synthesis was carried out using solutions of reducing agent sodium borohydride (NaBH4), silver nitrate (AgNO3) and collagen. Characterization was done by using dynamic light scattering (DLS) and X-ray diffraction (XRD) and AFM. Cellular viability testing was performed by using flow cytometry in human melanoma cancer (MV3) and murine fibroblast (L929) cells. The skin permeation study was conducted using a Franz diffusion cell, and the efficiency of AgNPcol against the formation of paw edema in mice was evaluated. The hydrodynamic diameter and zeta potential of AgNPcol were 140.7 +/- 7.8 nm and 20.1 +/- 0.7 mV, respectively. AgNPcol failed to induce early apoptosis, late apoptosis, and necrosis in L929 cells; however, it exhibited enhanced toxicity in cancer cells (MV3) compared to normal cells (1929). AgNPcol demonstrated increased toxicological effects in cancer MV3 cells, promoting skin permeation, and preventing paw edema. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/10267-0 - Structural chracterization of Carvacryl Acetate nanoparticles with schistosomicial application
• Grantee: Larissa Fernandes Batista
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/02282-6 - Structure-activity relationship studies about of Pilocarpus microphyllus (Rutaceae) alkaloid derivatives against Schistosoma mansoni
• Grantee: Ana Carolina Mafud
• Support Opportunities: Scholarships in Brazil - Post-Doctoral