Ageing is not associated with an altered immune response during Trypanosoma cruzi infection Ageing and Trypanosoma cruzi infection

Author(s)

Colato, Rafaela Pravato ; Brazao, Vania ; Santello, Fabricia Helena ; Alonso Toldo, Miriam Paula ; do Vale, Gabriel Tavares ; Tirapelli, Carlos Renato ; Pereira-da-Silva, Gabriela ; do Prado, Jr., Jose Clovis

• Document type: Journal article
• Source: Experimental Gerontology; v. 90, p. 43-51, APR 2017.
• Web of Science Citations: 2

Abstract

The aims of this work were to evaluate the influence of ageing on the magnitude of the immune response in male Wistar rats infected with the Y strain of Trypanosoma cruzi (T.cruzi). Infected young animals displayed enhanced CD4(+) T cells as compared to uninfected counterparts. Ageing also triggered a significant reduction in CD8(+) T cells compared to young and uninfected groups. The percentage of spleen NKT cells was reduced for all groups, regardless of the infection status. Significant decreased B-cells was noted in aged controls and infected animals as compared to young counterparts. A significant decrease in MHC class II (RT1B) expression in all aged animals was observed, whether infected or not. The highest and significant levels of Thiobarbituric Acid Reactive Substances (TBARS) were noted in the aged and infected animals as compared to young-infected ones (16 day). Consequently superoxide dismutase (SOD) activity was reduced for both control and infected aged animals. Significant elevation of 8-isoprostane levels was found in aged control and infected animals. Plasma glutathione (GSH) concentration was reduced in aged control animals, as well as, in the young infected animals. NO production was increased in both infected and uninfected aged animals compared to young infected and uninfected animals. Corticosterone levels were elevated in aged animals, whether infected or not. Thus, our results are inedited since the immune response is not worsened by the simple fact of animals being older. Ageing by itself triggered a damaged immune response as well as enhanced reactive oxygen species, when compared to young counterparts, but it did not contribute to impair the immune response of T. cruzi infected and aged rats. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/00303-6 - Immunosenescence: impact against the experimental infection with Trypanosoma cruzi
• Grantee: Rafaela Pravato Colato
• Support Opportunities: Scholarships in Brazil - Doctorate