| Full text | |
| Author(s): |
de Souza Melo, Carina Guimaraes
;
Colombo Martins Perles, Juliana Vanessa
;
Zanoni, Jacqueline Nelisis
;
Garcia de Souza, Sara Raquel
;
Santos, Erika Xavier
;
Leite, Aline de Lima
;
Heubel, Alessandro Domingues
;
Oliveira E Souza, Camila
;
de Souza, Juliana Gadelha
;
Rabelo Buzalaf, Marilia Afonso
Total Authors: 10
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 7, APR 21 2017. |
| Web of Science Citations: | 8 |
| Abstract | |
Ingested fluoride (F) is absorbed mainly in the small intestine, which is controlled by the Enteric Nervous System (ENS). Although important intestinal symptomatology has been described after excessive F exposure, there have been no studies reporting the effects of F on the ENS. In this study, the effects of chronic F exposure were evaluated on the duodenums of rats through proteomic and morphological analyses. Concentrations of 0, 10, or 50 ppm of F were applied to the drinking water for 30 days. Immunofluorescence techniques were performed in the myenteric plexus of the duodenum to detect HuC/D, neuronal nitric oxide (nNOS), vasoactive intestinal peptide (VIP), calcitonin gene related peptide (CGRP), and substance P (SP). The 50 ppm F group presented a significant decrease in the density of nNOS-IR neurons. Significant morphological alterations were also observed in HUC/D-IR and nNOS-IR neurons; VIP-IR, CGRP-IR, and SP-IR varicosities for both groups (10 and 50 ppm F). Proteomic analysis of the duodenum demonstrated alterations in the expression of several proteins, especially those related to important biological processes, such as protein polymerization, which helps to explain the downregulation of many proteins upon exposure to 50 ppm of F. (AU) | |
| FAPESP's process: | 11/10233-7 - Evaluation of enteric innervation and proteomic analysis of small intestin of rats exposed to chronic or acute fluoride dose |
| Grantee: | Carina Guimarães de Souza Melo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/16840-5 - Fluoride toxicology: proteomic studies in gut and liver |
| Grantee: | Marília Afonso Rabelo Buzalaf |
| Support Opportunities: | Regular Research Grants |