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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Novel Superoxide Anion Biosensor for Monitoring Reactive Species of Oxygen Released by Cancer Cells

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Author(s):
Crulhas, Bruno P. ; Recco, Lucas C. ; Delella, Flavia K. ; Pedrosa, Valber A.
Total Authors: 4
Document type: Journal article
Source: Electroanalysis; v. 29, n. 5, p. 1252-1257, MAY 2017.
Web of Science Citations: 6
Abstract

In this study, novel sensitive and selective hydrogel microstructures to detect superoxide anions released by cancer cells, based on electrochemical biosensors, are proposed. Ferrocene was coupled with superoxide dismutase within a poly(ethylene glycol) diacrylate hydrogel matrix. The pre-polymer solution was patterned by photolithography in gold microelectrodes fabricated on top of glass slides. The biosensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry, and was able to detect superoxide anions in a wide linear range from 5to 100 M, with a low detection limit of 0.001M and sensitivity of 14.1 nA M/mm(2). Moreover, the biosensor was able to directly detect reactive oxygen species released from prostate cells. Furthermore, the reproducibility, stability and selectivity of the biosensor achieved better results when compared with the previous report, so this methodology can be used in physiological and pathological detection of reactive oxygen species, providing a powerful platform for clinical diagnostics in the future. (AU)

FAPESP's process: 16/15919-8 - Monitoring of prostate cancer biomarkers using a biosensor
Grantee:Valber de Albuquerque Pedrosa
Support Opportunities: Regular Research Grants