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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Shotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharide

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Author(s):
Nogueira de Moraes, Carolina ; Maia, Leandro ; de Oliveira, Eliandre ; de Paula Freitas Dell'Aqua, Camila ; Chapwanya, Aspinas ; da Cruz Landim-Alvarenga, Fernando ; Oba, Eunice
Total Authors: 7
Document type: Journal article
Source: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 187, p. 42-47, MAY 2017.
Web of Science Citations: 2
Abstract

The use of the conditioned medium (CM) for diseases treatment is based on its enrichment with biomolecules with therapeutic properties and themselves have a beneficial effect. Secretome of bovine endometrial mesenchymal progenitor/stem cells (eMSCs) using a proteomics approach is until now unknown. This work aimed to evaluate the secretome of bovine eMSCs-CM challenged or not with lipopolysaccharide (LPS). For this, eMSCs characterized were challenged (TG) or not (CG). The CM was collected 12 h after stimulation and submitted to mass spectrometry analysis. The classification of identified proteins was done by PANTHER according to biological processes, molecular function, cellular component and protein class. 397 protein groups were identified in TG and 302 in CG. We observed positive enrichment for antibacterial response proteins, macrophage activation function, receptor-mediated endocytosis, hydrolase activity, inhibitory enzyme in TG, and for activity structural molecule and intermediate filament cytoskeleton in the CG. Our experimental model shows that eMSCs respond to LPS in the concentration used and can be used to study immune-inflammatory response, besides of the secretion of proteins mainly related to tissue remodeling, immune response and angiogenesis which is an interesting feature for use in cell therapy. (AU)

FAPESP's process: 14/20447-2 - Comparative study of the immunophenotypic profile, differentiation potential, capacity of production of cytocines and cryopreservation of endometrial stromal cells of cattle during the oestrous cycle
Grantee:Carolina Nogueira de Moraes Maia
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/18964-1 - Research training in characterization, cryopreservation, sorting, homing and gene therapy using MSCs in experimental models: evaluation of cytokine therapy using TRAIL associated to MSCs for the treatment of glioblastoma on animal model
Grantee:Carolina Nogueira de Moraes Maia
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 15/01057-1 - COMPARATIVE STUDY OF DIFFERENTIATION POTENTIAL, IMMUNOMODULATORY ACTIVITY AND CRYOPRESERVATION OF STROMAL CELL OF ENDOMETRIUM FROM COWS DURING DIFFERENT MOMENTS OF LUTEAL PHASE
Grantee:Eunice Oba
Support type: Regular Research Grants