Role of the locus coeruleus catecholaminergic neurons in the chemosensory control of breathing in a Parkinson's disease model

Author(s)

Oliveira, Luiz M. ; Tuppy, Marina ; Moreira, Thiago S. ; Takakura, Ana C.

• Document type: Journal article
• Source: Experimental Neurology; v. 293, p. 172-180, JUL 2017.
• Web of Science Citations: 14

Abstract

A previous study has demonstrated that in the 6-hydroxydopamine (6-OHDA)-model of Parkinson's disease (PD) there is a reduction in the number of Phox2b neurons in the retrotrapezoid nucleus (RTN) and a decrease in the respiratory response to hypercapnia 40 days after PD-induction. The functional deficiency is restored 60 days after 6-0HDA injection and here we tested the hypothesis that the locus coeruleus (LC) could be a candidate to restore the breathing deficiency. Minute Ventilation (V-E) in response to hypercapnia (7% CO2) was assessed one day before, and then 40 and 60 days after bilateral 6-0HDA (24 mu g/mu L) or vehicle injections into the LC in control or PD-induced male Wistar rats. Bilateral injections of 6-0HDA decreased catecholaminergic neurons by 86% and 83% in the substantia nigra pars compacta (SNpc) and LC, respectively. As already described, in animals with lesions to the SNpc (N = 6/group), the reduction in the ventilatory response to hypercapnia was restored 60 days after PD (1257 +/- 81 vs. vehicle: 1185 +/- 49 mL/kg/min). However, in animals with PD and lesion in the LC, the ventilation was blunted (674 39 mL/kg/min). In another group of PD rats, we observed a reduction in the number of hypercapnia-induced-fos(+) cells in the RTN region (40 days: 38 +/- 3 and 60 days: 8.5 +/- 0.9 vs. vehicle 78 +/- 3 cells) and an increase in the LC (40 days: 46 +/- 4 and 60 days: 94 +/- 22 vs. vehicle 1 +/- 1 cells). Our data suggest that LC catecholaminergic neurons can be a candidate structure mediating chemoreceptor function in a model of PD. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/22406-1 - Respiratory anatomofunctional changes observed in an experimental model of Parkinson Disease
• Grantee: Ana Carolina Thomaz Takakura
• Support type: Regular Research Grants
• FAPESP's process: 16/23281-3 - Encephalic regions responsible for neuroplasticity observed in respiratory response induced by hypercapnia in a modelo of Parkinson´s s disease
• Grantee: Ana Carolina Thomaz Takakura
• Support type: Regular Research Grants
• FAPESP's process: 15/23376-1 - Retrotrapezoid nucleus, respiratory chemosensitivity and breathing automaticity
• Grantee: Thiago dos Santos Moreira
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 15/18842-3 - Orexinergic and aminergic mechanisms in respiratory control in an animal model of Parkinsons Disease
• Grantee: Luiz Marcelo Oliveira Santos
• Support type: Scholarships in Brazil - Doctorate