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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the immature platelet fraction contribute to the differential diagnosis of hereditary, immune and other acquired thrombocytopenias

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Ferreira, F. L. B. ; Colella, M. P. ; Medina, S. S. ; Costa-Lima, C. ; Fiusa, M. M. L. ; Costa, L. N. G. ; Orsi, F. A. ; Annichino-Bizzacchi, J. M. ; Fertrin, K. Y. ; Gilberti, M. F. P. ; Ozelo, M. C. ; De Paula, E. V.
Total Authors: 12
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, JUN 13 2017.
Web of Science Citations: 8
Abstract

The differential diagnosis of immune (ITP) and hereditary macrothrombocytopenia (HM) is key to patient management. The immature platelet fraction (IPF) represents the subset of circulating platelets with higher RNA content, and has been shown to distinguish hypo-from hyperproliferative thrombocytopenias. Here we evaluated the diagnostic accuracy of IPF in the differential diagnosis between HM and other thrombocytopenias in a population of patients with post-chemotherapy thrombocytopenia (n = 56), bone marrow failure (n = 22), ITP (n = 105) and HM (n = 27). TPO levels were also measured in HM and ITP matched for platelet counts. Platelet counts were similar in all patient groups. Higher IPF values were observed in both ITP (12.3%; 2.4-65.6%) and HM (29.8%; 4.6-65.9%) compared to hypoproliferative thrombocytopenias. IPF values were also higher in HM compared to ITP, yielding a diagnostic accuracy of 0.80 (95%CI 0.70-0.90; P < 0.0001) to distinguish these two conditions. Intra-and inter-assays reproducibility of IPF in HM patients revealed that this is a stable parameter. In conclusion, IPF is increased in HM compared to both ITP and other thrombocytopenias and contributes to the differentiation between ITP and HM. Further studies are warranted to understand the biological rationale of these findings and to its incorporation in diagnostic algorithms of HM. (AU)

FAPESP's process: 13/09319-0 - Exploration of new mechanisms of epithelial barrier brekdown in acute gastrintestinal graft-versus-host-disease associated with alterations in "tight junctions"
Grantee:Erich Vinicius de Paula
Support Opportunities: Regular Research Grants