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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares

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Author(s):
Silva, E. S. M. ; Ignacio, F. S. ; Fritsch, S. C. ; Zanoni, D. S. ; Pantoja, J. C. F. ; Oliveira-Filho, J. P. ; Meira, C.
Total Authors: 7
Document type: Journal article
Source: Theriogenology; v. 97, p. 159-169, JUL 15 2017.
Web of Science Citations: 4
Abstract

To test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ER alpha and ER beta) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ER alpha and ER beta when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares' five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ER alpha and ER beta transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 12/00298-7 - Qualitative and quantitative evaluation of estrogen and progesterone receptors in non-cyclic mares treated with estradiol benzoate and Altrenogest
Grantee:Elisa Sant'Anna Monteiro da Silva
Support Opportunities: Scholarships in Brazil - Doctorate