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Hormonal analysis, immunolocalization and quantification of the androgen and estrogen receptors in the ovaries of rats submitted to different doses of nandrolone decanoate: assessment in the post-treatment and post-recovery periods

Grant number: 13/14510-0
Support Opportunities:Regular Research Grants
Duration: October 01, 2013 - September 30, 2015
Field of knowledge:Biological Sciences - Morphology - Embryology
Principal Investigator:Isabel Cristina Cherici Camargo
Grantee:Isabel Cristina Cherici Camargo
Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
Associated researchers:Luiz Gustavo de Almeida Chuffa

Abstract

Although the clinical application of anabolic androgenic steroids (AAS) is recommended for postmenopausal hormone replacement therapy and treatment of sarcopenia and cachexia associated with AIDS, renal failure, cancer and severe burns, many people in modern society have made use of these drugs by aesthetic reasons. This consumption has increased among women in recent decades. It is widely reported that AAS promotes adverse effects on reproduction. However, little attention is given about the effects promoted by AAS in the morphology of the female reproductive tract, and whether these effects are reversible. No studies have evaluated different doses of synthetic steroids administered to the females and the possibility of reversibility of side effects. Thus, the aim of this work is to evaluate the effect of increasing doses of the steroid nandrolone decanoate (1.87, 3.75, 7.5 and 15.0 mg / kg body weight) in the estrous cycle and ovarian tissue of albino rats, and whether a possible recovery of reproductive damage after cessation of steroid treatment. Each experimental group will be composed of 12 females. The control group will receive mineral oil. The treatments will performed by a single subcutaneous injection daily for 15 consecutive days. After the treatment period, six rats for each group will be euthanized and the other six will be submitted to a recovery period of 30 consecutive days without any treatment. The estrous cycle will be monitored daily. At the end of each evaluation period, the reproductive organs will be collected, weighed and the ovaries prepared for immunohistochemical analysis and Western Blot. Will also be evaluated serum concentrations of FSH, LH, Estradiol, Progesterone, Testosterone, DHT and SHBG. The results allow to compare the effects of dose and time-dependent promoted by nandrolone decanoate in gonadal tissue of rats and contribute to the understanding of the molecular mechanisms involved in hormone-receptor interaction in each of the study periods (post-treatment and post-recovery) . (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SIMAO, VINICIUS AUGUSTO; LUPI JUNIOR, LUIZ ANTONIO; ARAUJO LEITE, GABRIEL ADAN; CHERICI CAMARGO, ISABEL CRISTINA; DE ALMEIDA CHUFFA, LUIZ GUSTAVO. androlone decanoate causes uterine injury by changing hormone levels and sex steroid receptors in a dose- and time-dependent manne. REPRODUCTIVE TOXICOLOGY, v. 102, p. 98-108, . (12/01747-0, 13/14510-0)
SIMAO, VINICIUS AUGUSTO; DE ALMEIDA CHUFFA, LUIZ GUSTAVO; CHERICI CAMARGO, ISABEL CRISTINA. Ovarian sex steroid receptors and sex hormones in androgenized rats. Reproduction, v. 152, n. 5, p. 545-559, . (13/14510-0, 12/01747-0)
SIMAO, VINICIUS AUGUSTO; BELARDIN, LARISSA BERLOFFA; ARAUJO LEITE, GABRIEL ADAN; DE ALMEIDA CHUFFA, LUIZ GUSTAVO; CHERICI CAMARGO, ISABEL CRISTINA. Effects of different doses of nandrolone decanoate on estrous cycle and ovarian tissue of rats after treatment and recovery periods. International Journal of Experimental Pathology, v. 96, n. 5, p. 338-349, . (13/14510-0, 12/01747-0, 12/03813-0)

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