The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time

Author(s)

Aranda Salomao, Priscila Maria ; de Oliveira, Flavia Amadeu ; Rodrigues, Paula Danielle ; Al-Ahj, Luana Polioni ; da Silva Gasque, Kellen Cristina ; Jeggle, Pia ; Rabelo Buzalaf, Marilia Afonso ; de Oliveira, Rodrigo Cardoso ; Edwardson, John Michael ; Magalhaes, Ana Carolina

• Document type: Journal article
• Source: PLoS One; v. 12, n. 6 JUN 14 2017.
• Web of Science Citations: 6

Abstract

Objective Titanium tetrafluoride (TiF4) has shown promising effect in preventing tooth lesions. Therefore, we compared the cytotoxicity of TiF4 with sodium fluoride (NaF) (already applied in Dentistry) considering different fluoride concentrations, pH values and experimental models. Materials and methods Step 1) NIH/3T3 fibroblasts were exposed to mediums containing NaF or TiF4 (from 0.15 to 2.45% F), both at native and adjusted pH, for 6 h. Step 2) NIH/3T3 were exposed to NaF or TiF4 varnishes with 0.95, 1.95 or 2.45% F (native pH), for 6, 12 or 24 h. We applied MTT (1st and 2nd steps) and Hoescht/PI stain (2nd step) assays. Step 3) NIH/3T3 were exposed to NaF or TiF4 varnish (2.45% F), at native pH, for 6 or 12 h. The cell stiffness was measured by atomic force microscopy (AFM). Results Step 1) All cells exposed to NaF or TiF4 mediums died, regardless of the F concentration and pH. Step 2) Both varnishes, at 1.90 and 2.45% F, reduced cell viability by similar extents (33-86% at 6 h, 35-93% at 12 h, and 87-98% at 24 h) compared with control, regardless of the type of fluoride. Varnishes with 0.95% F did not differ from control. Step 3) TiF4 and NaF reduced cell stiffness to a similar extent, but only TiF4 differed from control at 6 h. Conclusions Based on the results of the 3 experimental steps, we conclude that TiF4 and NaF have similar cytotoxicity. The cytotoxicity was dependent on F concentration and exposure time. This result gives support for testing the effect of TiF4 varnish in vivo. (AU)

• FAPESP's process: 13/20372-0 - Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay, morphological analysis and cell signals for apoptotic pathways
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/16415-8 - Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay and cell death
• Grantee: Paula Danielle Rodrigues
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/17360-4 - Effect of Titanium Tetrafluoride on viability and morphology of NIH3T3 fibroblasts
• Grantee: Luana Polioni Al-Ahj
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/05009-1 - Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay, morphological analysis and cell signals for apoptotic pathway
• Grantee: Priscila Maria Aranda Salomão
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 10/14984-4 - EFFECT OF FLUORIDE SALTS ON THE VIABILITY OF FIBROBLASTS AND PRE-OSTEOBLASTS
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/13484-6 - Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay, morphological analysis and cell signals for apoptotic pathways
• Grantee: Priscila Maria Aranda Salomão
• Support Opportunities: Scholarships in Brazil - Doctorate