Bromelain Loading and Release from a Hydrogel Formulated Using Alginate and Arabic Gum

Author(s)

Ataide, Janaina Artem ; Cefali, Leticia Caramori ; Rebelo, Marcia de Araujo ; Spir, Livia Genovez ; Tambourgi, Elias Basile ; Jozala, Angela Faustino ; Chaud, Marco Vinicius ; Silveira, Edgar ; Gu, Xiaochen ; Mazzola, Priscila Gava

• Document type: Journal article
• Source: Planta Medica; v. 83, n. 10, p. 870-876, JUL 2017.
• Web of Science Citations: 6

Abstract

An ideal wound dressing ensures a moist environment around the wound area and absorbs exudates from the wound surface. Topical application of bromelain to incised wounds has been shown to reprogram the wound microenvironment to promote effective tissue repair. Combining the characteristics of hydrogels and bromelain is therefore of great interest. Herein, we describe the development of a hydrogel, formulated using alginate and Arabic gum, for bromelain loading and release. The hydrogel formulation was evaluated using response surface methodology, considering the pH value and the concentration of alginate and Arabic gum. Bromelain loading and release were evaluated based on passive diffusion. Differential scanning calorimetry and Fourier transform infrared spectroscopy were performed to confirm bromelain immobilization in the hydrogel. The final hydrogel formulation had a swelling ratio of 227% and incorporated 19% of bromelain from a bromelain solution. Bromelain immobilization in the hydrogel was the result of hydrogen bond formation and was optimal at 4 degrees C after 4 h of contact. This evidence suggests that bromelain entrapment into a hydrogel is a promising strategy for the development of wound dressings that support the debridement of burns and wounds. (AU)

• FAPESP's process: 11/23076-7 - Bromelain extraction from pineapple residues using ethanol precipitation and its application in polymeric hydrogel
• Grantee: Janaína Artem Ataide
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/26304-9 - Evaluation of skin permeation of bromelain using in vitro diffusion experiment
• Grantee: Janaína Artem Ataide
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation