Blood and milk polymorphonuclear leukocyte and monocyte/macrophage functions in naturally caprine arthritis encephalitis virus infection in dairy goats

Author(s)

Santos, Bruna Parapinski ; Souza, Fernando Nogueira ; Blagitz, Maiara Garcia ; Batista, Camila Freitas ; Bertagnon, Heloisa Godoi ; Diniz, Soraia Araujo ; Silva, Marcos Xavier ; Amaral Haddad, Joao Paulo ; Paiva Della Libera, Alice Maria Melville

• Document type: Journal article
• Source: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 188, p. 21-26, JUN 2017.
• Web of Science Citations: 1

Abstract

The exact influence of caprine arthritis encephalitis virus (CAEV) infection on blood and milk polymorphonuclear leukocytes (PMNLs) and monocyte/macrophages of goats remains unclear. Thus, the present study sought to explore the blood and milk PMNL and monocyte/macrophage functions in naturally CAEV-infected goats. The present study used 18 healthy Saanen goats that were segregated according to sera test outcomes into serologically CAEV negative (n = 8; 14 halves) and positive (n = 10; 14 halves) groups. All milk samples from mammary halves with milk bacteriologically positive outcomes, somatic cell count >= 2 x 10(6) cells mL(-1), and abnormal secretions in the strip cup test were excluded. We evaluated the percentage of blood and milk PMNLs and monocyte/macrophages, the viability of PMNLs and monocyte/macrophages, the levels of intracellular reactive oxygen species (ROS) and the nonopsonized phagocytosis of Staphylococcus aureus and Escherichia coli by flow cytometry. In the present study, a higher percentage of milk macrophages (CD14(+)) and milk polymorphonuclear leukocytes undergoing late apoptosis or necrosis (Annexin-V+/Propidium iodide(+)) was observed in CAEV-infected goats; we did not find any further alterations in blood and milk PMNL and monocyte/macrophage functions. Thus, regarding our results, the goats naturally infected with CAEV did not reveal pronounced dysfunctions in blood and milk polymorphonuclear leukocytes and monocytes/macrophages. (AU)

• FAPESP's process: 09/13410-7 - Mammary gland immunogram of goats naturally infected with Caprine Encephalitis Arthritis
• Grantee: Bruna Parapinski dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/07716-3 - Influence of caprine arthritis encephalitis virus on blood and milk immunogram ofgoats naturally infected
• Grantee: Alice Maria Melville Paiva Della Libera
• Support Opportunities: Regular Research Grants