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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Potential application of native lipases in the resolution of (RS) - phenylethylamine

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Author(s):
Cristiane Pilissão [1] ; Patrícia de O. Carvalho [2] ; Maria da G. Nascimento [3]
Total Authors: 3
Affiliation:
[1] Universidade Federal de Santa Catarina. Departamento de Química - Brasil
[2] Universidade São Francisco. Curso de Farmácia - Brasil
[3] Universidade Federal de Santa Catarina. Departamento de Química - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 21, n. 6, p. 973-977, 2010-00-00.
Abstract

The performance of two native lipases (lipase from Aspergillus niger and Rhizoupus oligosporus) in the resolution of (RS)-phenylethylamine (1), varying the temperature, acyl donor type (ethyl acetate, vinyl acetate, iso-propenyl acetate and acetic anhydride) and organic medium, was studied. The effect of the nature of the anion using native A. niger lipase in n-heptane with a series of imidazolium-based ILs [BMIm][X], where X = BF4, PF6, SCN and Cl, was also evaluated. Using the lipase from A. niger, the R-2b amide was obtained with conversions from 6 to > 99% and E-values from 2 to > 200, with n-heptane or n-hexane. This lipase showed better E-values in a two-phase system using n-heptane and [BMIm][PF6] or [BMIm][BF4] 9:1 (v/v), obtaining values of 9 and 7, respectively, when vinyl acetate was used as the acyl donor, compared to the use of pure n-heptane (E = 2). The series for the anions in terms of decreasing performance was as follows: PF6- >BF4- > SCN- > Cl-. (AU)