Influence of different culture media on the growth of Pfaffia glomerata (Spreng.) Pendersen (Amaranthaceae) for conservation in vitro

Author(s)

R.B.N. Alves ^[1] ; B.W. Bertoni ^[2] ; R.F. Vieira ^[3] ; S.C. França ^[4] ; L.C. Ming ^[5] ; Am.S. Pereira ^[6]

Affiliation

^[1] Embrapa Recursos Genéticos e Biotecnologia - Brasil

^[2] Universidade de Ribeirão Preto - Brasil

^[3] Embrapa Recursos Genéticos e Biotecnologia - Brasil

^[4] Universidade de Ribeirão Preto - Brasil

^[5] Unesp. FCA. Departamento de Horticultura - Brasil

^[6] Unesp. FCA. Departamento de Horticultura - Brasil

• Document type: Journal article
• Source: Revista Brasileira de Plantas Medicinais; v. 12, n. 4, p. 510-515, 2010-12-00.

Abstract

Pfaffia glomerata occurs in several states of Brazil and its neighboring countries in the south region at riverbanks and gallery forests. It is a hydrophyte and heliophyte species. The roots of the genus Pfaffia are used in Brazilian folk medicine especially as tonic, aphrodisiac and to control diabetes. The aim of this work was to establish an in vitro germplasm bank for Pfaffia glomerata. The experiment was carried out in completely randomized design with six treatments: 1) DM + 2% sucrose + 4% sorbitol; 2) DM/2 + 2% sucrose + 4% sorbitol; 3) DM + 2% sucrose + 4% sorbitol + 2 mg L-1 calcium pantothenate; 4) DM/2 + 2% sucrose + 4% sorbitol + 2 mg L-1 calcium pantothenate; 5) DM + 2% sucrose + 3% mannitol + 2 mg L-1 calcium pantothenate; 6) DM/2 + 2% sucrose + 3% mannitol + 2 mg L-1 calcium pantothenate. Results were subjected to analysis of variance and Scott Knott test for mean grouping. Treatments 1, 3 and 4 had a significantly larger number of nodal segments per stem, compared to Treatments 2, 5 and 6. Treatment 2 was the most appropriate for the in vitro conservation of this species since it led to the lowest growth (3.1±1.9 cm height), high survival rate, 100% explants with sprouting, and the largest number of sprouts per explant after six months of culture. All seedlings produced root and showed no formation of calluses or hyperhydricidity under the evaluated treatments. Acclimatized plants showed 100% survival in the ex vitro environment. Maintaining P. glomerata accessions in an in vitro germplasm bank is viable both economically and for conservation. (AU)